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Expression and Characterization of Coprothermobacter proteolyticus Alkaline Serine Protease

A putative protease gene (aprE) from the thermophilic bacterium Coprothermobacter proteolyticus was cloned and expressed in Bacillus subtilis. The enzyme was determined to be a serine protease based on inhibition by PMSF. Biochemical characterization demonstrated that the enzyme had optimal activity...

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Detalles Bibliográficos
Autores principales: Majeed, Tanveer, Tabassum, Romana, Orts, William J., Lee, Charles C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3886229/
https://www.ncbi.nlm.nih.gov/pubmed/24453843
http://dx.doi.org/10.1155/2013/396156