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Competitive cobalt for zinc substitution in mammalian methionine sulfoxide reductase B1 overexpressed in E. coli: structural and functional insight

Expression of the mammalian enzyme methionine sulfoxide reductase B1 (MsrB1) in Escherichia coli growing in cobalt-containing media resulted in the reproducible appearance of the stable cobalt-containing protein MsrB1-Co. NMR studies and biocomputing using the programs AnisoFit and Amber allowed us...

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Detalles Bibliográficos
Autores principales: Shumilina, Elena, Dobrovolska, Olena, Del Conte, Rebecca, Holen, Henrik Waldal, Dikiy, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3889830/
https://www.ncbi.nlm.nih.gov/pubmed/24271273
http://dx.doi.org/10.1007/s00775-013-1064-7