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Reduction of hydrogen peroxide stress derived from fatty acid beta-oxidation improves fatty acid utilization in Escherichia coli

Fatty acids are a promising raw material for substance production because of their highly reduced and anhydrous nature, which can provide higher fermentation yields than sugars. However, they are insoluble in water and are poorly utilized by microbes in industrial fermentation production. We used fa...

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Autores principales: Doi, Hidetaka, Hoshino, Yasushi, Nakase, Kentaro, Usuda, Yoshihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3890546/
https://www.ncbi.nlm.nih.gov/pubmed/24169950
http://dx.doi.org/10.1007/s00253-013-5327-6
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author Doi, Hidetaka
Hoshino, Yasushi
Nakase, Kentaro
Usuda, Yoshihiro
author_facet Doi, Hidetaka
Hoshino, Yasushi
Nakase, Kentaro
Usuda, Yoshihiro
author_sort Doi, Hidetaka
collection PubMed
description Fatty acids are a promising raw material for substance production because of their highly reduced and anhydrous nature, which can provide higher fermentation yields than sugars. However, they are insoluble in water and are poorly utilized by microbes in industrial fermentation production. We used fatty acids as raw materials for l-lysine fermentation by emulsification and improved the limited fatty acid-utilization ability of Escherichia coli. We obtained a fatty acid-utilizing mutant strain by laboratory evolution and demonstrated that it expressed lower levels of an oxidative-stress marker than wild type. The intracellular hydrogen peroxide (H(2)O(2)) concentration of a fatty acid-utilizing wild-type E. coli strain was higher than that of a glucose-utilizing wild-type E. coli strain. The novel mutation rpsA (D210Y) identified in our fatty acid-utilizing mutant strain enabled us to promote cell growth, fatty-acid utilization, and l-lysine production from fatty acid. Introduction of this rpsA (D210Y) mutation into a wild-type strain resulted in lower H(2)O(2) concentrations. The overexpression of superoxide dismutase (sodA) increased intracellular H(2)O(2) concentrations and inhibited E. coli fatty-acid utilization, whereas overexpression of an oxidative-stress regulator (oxyS) decreased intracellular H(2)O(2) concentrations and promoted E. coli fatty acid utilization and l-lysine production. Addition of the reactive oxygen species (ROS) scavenger thiourea promoted l-lysine production from fatty acids and decreased intracellular H(2)O(2) concentrations. Among the ROS generated by fatty-acid β-oxidation, H(2)O(2) critically affected E. coli growth and l-lysine production. This indicates that the regression of ROS stress promotes fatty acid utilization, which is beneficial for fatty acids used as raw materials in industrial production.
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spelling pubmed-38905462014-01-28 Reduction of hydrogen peroxide stress derived from fatty acid beta-oxidation improves fatty acid utilization in Escherichia coli Doi, Hidetaka Hoshino, Yasushi Nakase, Kentaro Usuda, Yoshihiro Appl Microbiol Biotechnol Biotechnological Products and Process Engineering Fatty acids are a promising raw material for substance production because of their highly reduced and anhydrous nature, which can provide higher fermentation yields than sugars. However, they are insoluble in water and are poorly utilized by microbes in industrial fermentation production. We used fatty acids as raw materials for l-lysine fermentation by emulsification and improved the limited fatty acid-utilization ability of Escherichia coli. We obtained a fatty acid-utilizing mutant strain by laboratory evolution and demonstrated that it expressed lower levels of an oxidative-stress marker than wild type. The intracellular hydrogen peroxide (H(2)O(2)) concentration of a fatty acid-utilizing wild-type E. coli strain was higher than that of a glucose-utilizing wild-type E. coli strain. The novel mutation rpsA (D210Y) identified in our fatty acid-utilizing mutant strain enabled us to promote cell growth, fatty-acid utilization, and l-lysine production from fatty acid. Introduction of this rpsA (D210Y) mutation into a wild-type strain resulted in lower H(2)O(2) concentrations. The overexpression of superoxide dismutase (sodA) increased intracellular H(2)O(2) concentrations and inhibited E. coli fatty-acid utilization, whereas overexpression of an oxidative-stress regulator (oxyS) decreased intracellular H(2)O(2) concentrations and promoted E. coli fatty acid utilization and l-lysine production. Addition of the reactive oxygen species (ROS) scavenger thiourea promoted l-lysine production from fatty acids and decreased intracellular H(2)O(2) concentrations. Among the ROS generated by fatty-acid β-oxidation, H(2)O(2) critically affected E. coli growth and l-lysine production. This indicates that the regression of ROS stress promotes fatty acid utilization, which is beneficial for fatty acids used as raw materials in industrial production. Springer Berlin Heidelberg 2013-10-30 2014 /pmc/articles/PMC3890546/ /pubmed/24169950 http://dx.doi.org/10.1007/s00253-013-5327-6 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by-nc/2.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Biotechnological Products and Process Engineering
Doi, Hidetaka
Hoshino, Yasushi
Nakase, Kentaro
Usuda, Yoshihiro
Reduction of hydrogen peroxide stress derived from fatty acid beta-oxidation improves fatty acid utilization in Escherichia coli
title Reduction of hydrogen peroxide stress derived from fatty acid beta-oxidation improves fatty acid utilization in Escherichia coli
title_full Reduction of hydrogen peroxide stress derived from fatty acid beta-oxidation improves fatty acid utilization in Escherichia coli
title_fullStr Reduction of hydrogen peroxide stress derived from fatty acid beta-oxidation improves fatty acid utilization in Escherichia coli
title_full_unstemmed Reduction of hydrogen peroxide stress derived from fatty acid beta-oxidation improves fatty acid utilization in Escherichia coli
title_short Reduction of hydrogen peroxide stress derived from fatty acid beta-oxidation improves fatty acid utilization in Escherichia coli
title_sort reduction of hydrogen peroxide stress derived from fatty acid beta-oxidation improves fatty acid utilization in escherichia coli
topic Biotechnological Products and Process Engineering
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3890546/
https://www.ncbi.nlm.nih.gov/pubmed/24169950
http://dx.doi.org/10.1007/s00253-013-5327-6
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