Cargando…
CD56(neg)CD16(+) NK cells are activated mature NK cells with impaired effector function during HIV-1 infection
BACKGROUND: A subset of CD3(neg)CD56(neg)CD16(+) Natural Killer (NK) cells is highly expanded during chronic HIV-1 infection. The role of this subset in HIV-1 pathogenesis remains unclear. The lack of NK cell lineage-specific markers has complicated the study of minor NK cell subpopulations. RESULTS...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3892122/ https://www.ncbi.nlm.nih.gov/pubmed/24351015 http://dx.doi.org/10.1186/1742-4690-10-158 |
_version_ | 1782299470666399744 |
---|---|
author | Milush, Jeffrey M López-Vergès, Sandra York, Vanessa A Deeks, Steven G Martin, Jeffrey N Hecht, Frederick M Lanier, Lewis L Nixon, Douglas F |
author_facet | Milush, Jeffrey M López-Vergès, Sandra York, Vanessa A Deeks, Steven G Martin, Jeffrey N Hecht, Frederick M Lanier, Lewis L Nixon, Douglas F |
author_sort | Milush, Jeffrey M |
collection | PubMed |
description | BACKGROUND: A subset of CD3(neg)CD56(neg)CD16(+) Natural Killer (NK) cells is highly expanded during chronic HIV-1 infection. The role of this subset in HIV-1 pathogenesis remains unclear. The lack of NK cell lineage-specific markers has complicated the study of minor NK cell subpopulations. RESULTS: Using CD7 as an additional NK cell marker, we found that CD3(neg)CD56(neg)CD16(+) cells are a heterogeneous population comprised of CD7(+) NK cells and CD7(neg) non-classical myeloid cells. CD7(+)CD56(neg)CD16(+) NK cells are significantly expanded in HIV-1 infection. CD7(+)CD56(neg)CD16(+) NK cells are mature and express KIRs, the C-type lectin-like receptors NKG2A and NKG2C, and natural cytotoxicity receptors similar to CD7(+)CD56(+)CD16(+) NK cells. CD7(+)CD56(neg) NK cells in healthy donors produced minimal IFNγ following K562 target cell or IL-12 plus IL-18 stimulation; however, they degranulated in response to K562 stimulation similar to CD7(+)CD56(+) NK cells. HIV-1 infection resulted in reduced IFNγ secretion following K562 or cytokine stimulation by both NK cell subsets compared to healthy donors. Decreased granzyme B and perforin expression and increased expression of CD107a in the absence of stimulation, particularly in HIV-1-infected subjects, suggest that CD7(+)CD56(neg)CD16(+) NK cells may have recently engaged target cells. Furthermore, CD7(+)CD56(neg)CD16(+) NK cells have significantly increased expression of CD95, a marker of NK cell activation. CONCLUSIONS: Taken together, CD7(+)CD56(neg)CD16(+) NK cells are activated, mature NK cells that may have recently engaged target cells. |
format | Online Article Text |
id | pubmed-3892122 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-38921222014-01-16 CD56(neg)CD16(+) NK cells are activated mature NK cells with impaired effector function during HIV-1 infection Milush, Jeffrey M López-Vergès, Sandra York, Vanessa A Deeks, Steven G Martin, Jeffrey N Hecht, Frederick M Lanier, Lewis L Nixon, Douglas F Retrovirology Research BACKGROUND: A subset of CD3(neg)CD56(neg)CD16(+) Natural Killer (NK) cells is highly expanded during chronic HIV-1 infection. The role of this subset in HIV-1 pathogenesis remains unclear. The lack of NK cell lineage-specific markers has complicated the study of minor NK cell subpopulations. RESULTS: Using CD7 as an additional NK cell marker, we found that CD3(neg)CD56(neg)CD16(+) cells are a heterogeneous population comprised of CD7(+) NK cells and CD7(neg) non-classical myeloid cells. CD7(+)CD56(neg)CD16(+) NK cells are significantly expanded in HIV-1 infection. CD7(+)CD56(neg)CD16(+) NK cells are mature and express KIRs, the C-type lectin-like receptors NKG2A and NKG2C, and natural cytotoxicity receptors similar to CD7(+)CD56(+)CD16(+) NK cells. CD7(+)CD56(neg) NK cells in healthy donors produced minimal IFNγ following K562 target cell or IL-12 plus IL-18 stimulation; however, they degranulated in response to K562 stimulation similar to CD7(+)CD56(+) NK cells. HIV-1 infection resulted in reduced IFNγ secretion following K562 or cytokine stimulation by both NK cell subsets compared to healthy donors. Decreased granzyme B and perforin expression and increased expression of CD107a in the absence of stimulation, particularly in HIV-1-infected subjects, suggest that CD7(+)CD56(neg)CD16(+) NK cells may have recently engaged target cells. Furthermore, CD7(+)CD56(neg)CD16(+) NK cells have significantly increased expression of CD95, a marker of NK cell activation. CONCLUSIONS: Taken together, CD7(+)CD56(neg)CD16(+) NK cells are activated, mature NK cells that may have recently engaged target cells. BioMed Central 2013-12-18 /pmc/articles/PMC3892122/ /pubmed/24351015 http://dx.doi.org/10.1186/1742-4690-10-158 Text en Copyright © 2013 Milush et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Milush, Jeffrey M López-Vergès, Sandra York, Vanessa A Deeks, Steven G Martin, Jeffrey N Hecht, Frederick M Lanier, Lewis L Nixon, Douglas F CD56(neg)CD16(+) NK cells are activated mature NK cells with impaired effector function during HIV-1 infection |
title | CD56(neg)CD16(+) NK cells are activated mature NK cells with impaired effector function during HIV-1 infection |
title_full | CD56(neg)CD16(+) NK cells are activated mature NK cells with impaired effector function during HIV-1 infection |
title_fullStr | CD56(neg)CD16(+) NK cells are activated mature NK cells with impaired effector function during HIV-1 infection |
title_full_unstemmed | CD56(neg)CD16(+) NK cells are activated mature NK cells with impaired effector function during HIV-1 infection |
title_short | CD56(neg)CD16(+) NK cells are activated mature NK cells with impaired effector function during HIV-1 infection |
title_sort | cd56(neg)cd16(+) nk cells are activated mature nk cells with impaired effector function during hiv-1 infection |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3892122/ https://www.ncbi.nlm.nih.gov/pubmed/24351015 http://dx.doi.org/10.1186/1742-4690-10-158 |
work_keys_str_mv | AT milushjeffreym cd56negcd16nkcellsareactivatedmaturenkcellswithimpairedeffectorfunctionduringhiv1infection AT lopezvergessandra cd56negcd16nkcellsareactivatedmaturenkcellswithimpairedeffectorfunctionduringhiv1infection AT yorkvanessaa cd56negcd16nkcellsareactivatedmaturenkcellswithimpairedeffectorfunctionduringhiv1infection AT deekssteveng cd56negcd16nkcellsareactivatedmaturenkcellswithimpairedeffectorfunctionduringhiv1infection AT martinjeffreyn cd56negcd16nkcellsareactivatedmaturenkcellswithimpairedeffectorfunctionduringhiv1infection AT hechtfrederickm cd56negcd16nkcellsareactivatedmaturenkcellswithimpairedeffectorfunctionduringhiv1infection AT lanierlewisl cd56negcd16nkcellsareactivatedmaturenkcellswithimpairedeffectorfunctionduringhiv1infection AT nixondouglasf cd56negcd16nkcellsareactivatedmaturenkcellswithimpairedeffectorfunctionduringhiv1infection |