Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9

We have applied the CRISPR/Cas9 system to Drosophila S2 cells to generate targeted genetic mutations in more than 85% of alleles. By targeting a constitutive exon of the AGO1 gene, we demonstrate homozygous mutation in up to 82% of cells, thereby allowing the study of genetic knockouts in a Drosophi...

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Detalles Bibliográficos
Autores principales: Bassett, Andrew R., Tibbit, Charlotte, Ponting, Chris P., Liu, Ji-Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3892159/
https://www.ncbi.nlm.nih.gov/pubmed/24326186
http://dx.doi.org/10.1242/bio.20137120

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3892159/
https://www.ncbi.nlm.nih.gov/pubmed/24326186
http://dx.doi.org/10.1242/bio.20137120

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