Dosage and Cell Line Dependent Inhibitory Effect of bFGF Supplement in Human Pluripotent Stem Cell Culture on Inactivated Human Mesenchymal Stem Cells

Many different culture systems have been developed for expanding human pluripotent stem cells (hESCs and hiPSCs). In general, 4–10 ng/ml of bFGF is supplemented in culture media in feeder-dependent systems regardless of feeder cell types, whereas in feeder-free systems, up to 100 ng/ml of bFGF is re...

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Detalles Bibliográficos
Autores principales: Quang, Tara, Marquez, Maribel, Blanco, Giselle, Zhao, Yuanxiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3895015/
https://www.ncbi.nlm.nih.gov/pubmed/24465853
http://dx.doi.org/10.1371/journal.pone.0086031

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3895015/
https://www.ncbi.nlm.nih.gov/pubmed/24465853
http://dx.doi.org/10.1371/journal.pone.0086031

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