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Physiological Normoxia and Absence of EGF Is Required for the Long-Term Propagation of Anterior Neural Precursors from Human Pluripotent Cells

Widespread use of human pluripotent stem cells (hPSCs) to study neuronal physiology and function is hindered by the ongoing need for specialist expertise in converting hPSCs to neural precursor cells (NPCs). Here, we describe a new methodology to generate cryo-preservable hPSC-derived NPCs that reta...

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Detalles Bibliográficos
Autores principales: Bilican, Bilada, Livesey, Matthew R., Haghi, Ghazal, Qiu, Jing, Burr, Karen, Siller, Rick, Hardingham, Giles E., Wyllie, David J. A., Chandran, Siddharthan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3895023/
https://www.ncbi.nlm.nih.gov/pubmed/24465796
http://dx.doi.org/10.1371/journal.pone.0085932