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Physiological Normoxia and Absence of EGF Is Required for the Long-Term Propagation of Anterior Neural Precursors from Human Pluripotent Cells
Widespread use of human pluripotent stem cells (hPSCs) to study neuronal physiology and function is hindered by the ongoing need for specialist expertise in converting hPSCs to neural precursor cells (NPCs). Here, we describe a new methodology to generate cryo-preservable hPSC-derived NPCs that reta...
Autores principales: | Bilican, Bilada, Livesey, Matthew R., Haghi, Ghazal, Qiu, Jing, Burr, Karen, Siller, Rick, Hardingham, Giles E., Wyllie, David J. A., Chandran, Siddharthan |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3895023/ https://www.ncbi.nlm.nih.gov/pubmed/24465796 http://dx.doi.org/10.1371/journal.pone.0085932 |
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