A new regulatory pathway of mRNA export by an F-box protein, Mdm30

Mdm30, an F-box protein in yeast, has been recently shown to promote mRNA export. However, it remains unknown how Mdm30 facilitates mRNA export. Here, we show that Mdm30 targets the Sub2 component of the TREX (Transcription/Export) complex for ubiquitylation and subsequent proteasomal degradation. S...

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Detalles Bibliográficos
Autores principales: Durairaj, Geetha, Lahudkar, Shweta, Bhaumik, Sukesh R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2014
Materias:
Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3895266/
https://www.ncbi.nlm.nih.gov/pubmed/24327750
http://dx.doi.org/10.1261/rna.042325.113
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author Durairaj, Geetha
Lahudkar, Shweta
Bhaumik, Sukesh R.
author_facet Durairaj, Geetha
Lahudkar, Shweta
Bhaumik, Sukesh R.
author_sort Durairaj, Geetha
collection PubMed
description Mdm30, an F-box protein in yeast, has been recently shown to promote mRNA export. However, it remains unknown how Mdm30 facilitates mRNA export. Here, we show that Mdm30 targets the Sub2 component of the TREX (Transcription/Export) complex for ubiquitylation and subsequent proteasomal degradation. Such a targeted degradation of Sub2 enhances the recruitment of the mRNA export adaptor, Yra1, to the active genes to promote mRNA export. Together, these results elucidate that Mdm30 promotes mRNA export by lowering Sub2's stability and consequently enhancing Yra1 recruitment, thus illuminating new regulatory mechanisms of mRNA export by Mdm30.
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spelling pubmed-38952662015-02-01 A new regulatory pathway of mRNA export by an F-box protein, Mdm30 Durairaj, Geetha Lahudkar, Shweta Bhaumik, Sukesh R. RNA Report Mdm30, an F-box protein in yeast, has been recently shown to promote mRNA export. However, it remains unknown how Mdm30 facilitates mRNA export. Here, we show that Mdm30 targets the Sub2 component of the TREX (Transcription/Export) complex for ubiquitylation and subsequent proteasomal degradation. Such a targeted degradation of Sub2 enhances the recruitment of the mRNA export adaptor, Yra1, to the active genes to promote mRNA export. Together, these results elucidate that Mdm30 promotes mRNA export by lowering Sub2's stability and consequently enhancing Yra1 recruitment, thus illuminating new regulatory mechanisms of mRNA export by Mdm30. Cold Spring Harbor Laboratory Press 2014-02 /pmc/articles/PMC3895266/ /pubmed/24327750 http://dx.doi.org/10.1261/rna.042325.113 Text en © 2014 Durairaj et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by-nc/3.0/ This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.
spellingShingle Report
Durairaj, Geetha
Lahudkar, Shweta
Bhaumik, Sukesh R.
A new regulatory pathway of mRNA export by an F-box protein, Mdm30
title A new regulatory pathway of mRNA export by an F-box protein, Mdm30
title_full A new regulatory pathway of mRNA export by an F-box protein, Mdm30
title_fullStr A new regulatory pathway of mRNA export by an F-box protein, Mdm30
title_full_unstemmed A new regulatory pathway of mRNA export by an F-box protein, Mdm30
title_short A new regulatory pathway of mRNA export by an F-box protein, Mdm30
title_sort new regulatory pathway of mrna export by an f-box protein, mdm30
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3895266/
https://www.ncbi.nlm.nih.gov/pubmed/24327750
http://dx.doi.org/10.1261/rna.042325.113
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