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The ‘Densitometric Image Analysis Software’ and Its Application to Determine Stepwise Equilibrium Constants from Electrophoretic Mobility Shift Assays

Current software applications for densitometric analysis, such as ImageJ, QuantityOne (BioRad) and the Intelligent or Advanced Quantifier (Bio Image) do not allow to take the non-linearity of autoradiographic films into account during calibration. As a consequence, quantification of autoradiographs...

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Detalles Bibliográficos
Autores principales: van Oeffelen, Liesbeth, Peeters, Eveline, Nguyen Le Minh, Phu, Charlier, Daniël
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897411/
https://www.ncbi.nlm.nih.gov/pubmed/24465496
http://dx.doi.org/10.1371/journal.pone.0085146