Imaging Mitochondrial Flux in Single Cells with a FRET Sensor for Pyruvate

Mitochondrial flux is currently accessible at low resolution. Here we introduce a genetically-encoded FRET sensor for pyruvate, and methods for quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resol...

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Detalles Bibliográficos
Autores principales: San Martín, Alejandro, Ceballo, Sebastián, Baeza-Lehnert, Felipe, Lerchundi, Rodrigo, Valdebenito, Rocío, Contreras-Baeza, Yasna, Alegría, Karin, Barros, L. Felipe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897509/
https://www.ncbi.nlm.nih.gov/pubmed/24465702
http://dx.doi.org/10.1371/journal.pone.0085780

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897509/
https://www.ncbi.nlm.nih.gov/pubmed/24465702
http://dx.doi.org/10.1371/journal.pone.0085780

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