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Observing Single Enzyme Molecules Interconvert between Activity States upon Heating

In this paper, we demonstrate that single enzyme molecules of β-galactosidase interconvert between different activity states upon exposure to short pulses of heat. We show that these changes in activity are the result of different enzyme conformations. Hundreds of single β-galactosidase molecules ar...

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Detalles Bibliográficos
Autores principales: Rojek, Marcin J., Walt, David R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897666/
https://www.ncbi.nlm.nih.gov/pubmed/24465972
http://dx.doi.org/10.1371/journal.pone.0086224