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Observing Single Enzyme Molecules Interconvert between Activity States upon Heating
In this paper, we demonstrate that single enzyme molecules of β-galactosidase interconvert between different activity states upon exposure to short pulses of heat. We show that these changes in activity are the result of different enzyme conformations. Hundreds of single β-galactosidase molecules ar...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2014
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897666/ https://www.ncbi.nlm.nih.gov/pubmed/24465972 http://dx.doi.org/10.1371/journal.pone.0086224 |