Characterization of cleavage intermediate and star sites of RM.Tth111II

Tth111II is a thermostable Type IIGS restriction enzyme that recognizes DNA sites CAARCA (R = A or G) and cleaves downstream at N11/N9. Here, the tth111IIRM gene was cloned and expressed in E. coli, and Tth111II was purified. The purified enzyme contains internally-bound S-adenosylmethionine (SAM)....

Descripción completa

Detalles Bibliográficos
Autores principales: Zhu, Zhenyu, Guan, Shengxi, Robinson, Derek, Fezzazi, Hanna El, Quimby, Aine, Xu, Shuang-yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3899748/
https://www.ncbi.nlm.nih.gov/pubmed/24452415
http://dx.doi.org/10.1038/srep03838

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3899748/
https://www.ncbi.nlm.nih.gov/pubmed/24452415
http://dx.doi.org/10.1038/srep03838

Ejemplares similares