In Vivo Evolution of a Catalytic RNA Couples Trans-Splicing to Translation

How does a non-coding RNA evolve in cells? To address this question experimentally we evolved a trans-splicing variant of the group I intron ribozyme from Tetrahymena over 21 cycles of evolution in E.coli cells. Sequence variation was introduced during the evolution by mutagenic and recombinative PC...

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Detalles Bibliográficos
Autores principales: Olson, Karen E., Dolan, Gregory F., Müller, Ulrich F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3900562/
https://www.ncbi.nlm.nih.gov/pubmed/24466112
http://dx.doi.org/10.1371/journal.pone.0086473

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3900562/
https://www.ncbi.nlm.nih.gov/pubmed/24466112
http://dx.doi.org/10.1371/journal.pone.0086473

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