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An Asp7Gly Substitution in PPARG Is Associated with Decreased Transcriptional Activation Activity
As the master regulator of adipogenesis, peroxisome proliferator-activated receptor gamma (PPARG) is required for the accumulation of adipose tissue and hence contributes to obesity. A previous study showed that the substitution of +20A>G in PPARG changed the 7(th) amino acid from Asp to Gly, cre...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3900691/ https://www.ncbi.nlm.nih.gov/pubmed/24466299 http://dx.doi.org/10.1371/journal.pone.0086954 |
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author | Hua, Liushuai Wang, Jing Li, Mingxun Sun, Xiaomei Zhang, Liangzhi Lei, Chuzhao Lan, Xianyong Fang, Xingtang Zhao, Xin Chen, Hong |
author_facet | Hua, Liushuai Wang, Jing Li, Mingxun Sun, Xiaomei Zhang, Liangzhi Lei, Chuzhao Lan, Xianyong Fang, Xingtang Zhao, Xin Chen, Hong |
author_sort | Hua, Liushuai |
collection | PubMed |
description | As the master regulator of adipogenesis, peroxisome proliferator-activated receptor gamma (PPARG) is required for the accumulation of adipose tissue and hence contributes to obesity. A previous study showed that the substitution of +20A>G in PPARG changed the 7(th) amino acid from Asp to Gly, creating a mutant referred to as PPARG Asp7Gly. In this study, association analysis indicated that PPARG Asp7Gly was associated with lower body height, body weight and heart girth in cattle (P<0.05). Overexpression of PPARG in NIH3T3-L1 cells showed that the Asp7Gly substitution may cause a decrease in its adipogenic ability and the mRNA levels of CIDEC (cell death-inducing DFFA-like effector c) and aP2, which are all transcriptionally activated by PPARG during adipocyte differentiation. A dual-luciferase reporter assay was used to analyze the promoter activity of CIDEC. The results confirmed that the mutant PPARG exhibited weaker transcriptional activation activity than the wild type (P<0.05). These findings likely explain the associations between the Asp7Gly substitution and the body measurements. Additionally, the Asp7Gly mutation may be used in molecular marker assisted selection (MAS) of cattle breeding in the future. |
format | Online Article Text |
id | pubmed-3900691 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39006912014-01-24 An Asp7Gly Substitution in PPARG Is Associated with Decreased Transcriptional Activation Activity Hua, Liushuai Wang, Jing Li, Mingxun Sun, Xiaomei Zhang, Liangzhi Lei, Chuzhao Lan, Xianyong Fang, Xingtang Zhao, Xin Chen, Hong PLoS One Research Article As the master regulator of adipogenesis, peroxisome proliferator-activated receptor gamma (PPARG) is required for the accumulation of adipose tissue and hence contributes to obesity. A previous study showed that the substitution of +20A>G in PPARG changed the 7(th) amino acid from Asp to Gly, creating a mutant referred to as PPARG Asp7Gly. In this study, association analysis indicated that PPARG Asp7Gly was associated with lower body height, body weight and heart girth in cattle (P<0.05). Overexpression of PPARG in NIH3T3-L1 cells showed that the Asp7Gly substitution may cause a decrease in its adipogenic ability and the mRNA levels of CIDEC (cell death-inducing DFFA-like effector c) and aP2, which are all transcriptionally activated by PPARG during adipocyte differentiation. A dual-luciferase reporter assay was used to analyze the promoter activity of CIDEC. The results confirmed that the mutant PPARG exhibited weaker transcriptional activation activity than the wild type (P<0.05). These findings likely explain the associations between the Asp7Gly substitution and the body measurements. Additionally, the Asp7Gly mutation may be used in molecular marker assisted selection (MAS) of cattle breeding in the future. Public Library of Science 2014-01-23 /pmc/articles/PMC3900691/ /pubmed/24466299 http://dx.doi.org/10.1371/journal.pone.0086954 Text en © 2014 Hua et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Hua, Liushuai Wang, Jing Li, Mingxun Sun, Xiaomei Zhang, Liangzhi Lei, Chuzhao Lan, Xianyong Fang, Xingtang Zhao, Xin Chen, Hong An Asp7Gly Substitution in PPARG Is Associated with Decreased Transcriptional Activation Activity |
title | An Asp7Gly Substitution in PPARG Is Associated with Decreased Transcriptional Activation Activity |
title_full | An Asp7Gly Substitution in PPARG Is Associated with Decreased Transcriptional Activation Activity |
title_fullStr | An Asp7Gly Substitution in PPARG Is Associated with Decreased Transcriptional Activation Activity |
title_full_unstemmed | An Asp7Gly Substitution in PPARG Is Associated with Decreased Transcriptional Activation Activity |
title_short | An Asp7Gly Substitution in PPARG Is Associated with Decreased Transcriptional Activation Activity |
title_sort | asp7gly substitution in pparg is associated with decreased transcriptional activation activity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3900691/ https://www.ncbi.nlm.nih.gov/pubmed/24466299 http://dx.doi.org/10.1371/journal.pone.0086954 |
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