Cargando…
Epigenetic silencing of ARRDC3 expression in basal-like breast cancer cells
Arrestin domain-containing 3 (ARRDC3) is a tumor suppressor whose expression is either lost or suppressed in basal-like breast cancer (BLBC). However, the mechanism by which BLBC suppresses ARRDC3 expression is not established. Here, we show that expression of ARRDC3 in BLBC cells is suppressed at t...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3900932/ https://www.ncbi.nlm.nih.gov/pubmed/24457910 http://dx.doi.org/10.1038/srep03846 |
_version_ | 1782300783778201600 |
---|---|
author | Soung, Young Hwa Pruitt, Kevin Chung, Jun |
author_facet | Soung, Young Hwa Pruitt, Kevin Chung, Jun |
author_sort | Soung, Young Hwa |
collection | PubMed |
description | Arrestin domain-containing 3 (ARRDC3) is a tumor suppressor whose expression is either lost or suppressed in basal-like breast cancer (BLBC). However, the mechanism by which BLBC suppresses ARRDC3 expression is not established. Here, we show that expression of ARRDC3 in BLBC cells is suppressed at the transcriptional level. Suppression of ARRDC3 expression in BLBC cells involves epigenetic silencing as inhibitors of class III histone deacetylases (HDACs) significantly restores ARRDC3 levels in BLBC cells. SIRT2, among class III HDACs, plays a major role in epigenetic silencing of ARRDC3 in MDA-MB-231 cells. Acetylation levels of the ARRDC3 promoter in BLBC cells is significantly lower than that of other sub-types of BC cells. Chromatin immunopreciptitation analysis established SIRT2 binding at ARRDC3 promoter in BLBC cells. Our studies indicate that SIRT2 dependent epigenetic silencing of ARRDC3 is one of the important events that may contribute to the aggressive nature of BLBC cells. |
format | Online Article Text |
id | pubmed-3900932 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-39009322014-01-24 Epigenetic silencing of ARRDC3 expression in basal-like breast cancer cells Soung, Young Hwa Pruitt, Kevin Chung, Jun Sci Rep Article Arrestin domain-containing 3 (ARRDC3) is a tumor suppressor whose expression is either lost or suppressed in basal-like breast cancer (BLBC). However, the mechanism by which BLBC suppresses ARRDC3 expression is not established. Here, we show that expression of ARRDC3 in BLBC cells is suppressed at the transcriptional level. Suppression of ARRDC3 expression in BLBC cells involves epigenetic silencing as inhibitors of class III histone deacetylases (HDACs) significantly restores ARRDC3 levels in BLBC cells. SIRT2, among class III HDACs, plays a major role in epigenetic silencing of ARRDC3 in MDA-MB-231 cells. Acetylation levels of the ARRDC3 promoter in BLBC cells is significantly lower than that of other sub-types of BC cells. Chromatin immunopreciptitation analysis established SIRT2 binding at ARRDC3 promoter in BLBC cells. Our studies indicate that SIRT2 dependent epigenetic silencing of ARRDC3 is one of the important events that may contribute to the aggressive nature of BLBC cells. Nature Publishing Group 2014-01-24 /pmc/articles/PMC3900932/ /pubmed/24457910 http://dx.doi.org/10.1038/srep03846 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Article Soung, Young Hwa Pruitt, Kevin Chung, Jun Epigenetic silencing of ARRDC3 expression in basal-like breast cancer cells |
title | Epigenetic silencing of ARRDC3 expression in basal-like breast cancer cells |
title_full | Epigenetic silencing of ARRDC3 expression in basal-like breast cancer cells |
title_fullStr | Epigenetic silencing of ARRDC3 expression in basal-like breast cancer cells |
title_full_unstemmed | Epigenetic silencing of ARRDC3 expression in basal-like breast cancer cells |
title_short | Epigenetic silencing of ARRDC3 expression in basal-like breast cancer cells |
title_sort | epigenetic silencing of arrdc3 expression in basal-like breast cancer cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3900932/ https://www.ncbi.nlm.nih.gov/pubmed/24457910 http://dx.doi.org/10.1038/srep03846 |
work_keys_str_mv | AT soungyounghwa epigeneticsilencingofarrdc3expressioninbasallikebreastcancercells AT pruittkevin epigeneticsilencingofarrdc3expressioninbasallikebreastcancercells AT chungjun epigeneticsilencingofarrdc3expressioninbasallikebreastcancercells |