Resting of Cryopreserved PBMC Does Not Generally Benefit the Performance of Antigen-Specific T Cell ELISPOT Assays

T cell monitoring is increasingly performed using cryopreserved PBMC. It has been suggested that resting of PBMC after thawing, that is, culturing them overnight in test medium, produces higher antigen-induced spot counts in ELISPOT assays. To evaluate the importance of overnight resting, we systema...

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Detalles Bibliográficos
Autores principales: Kuerten, Stefanie, Batoulis, Helena, Recks, Mascha S., Karacsony, Edith, Zhang, Wenji, Subbramanian, Ramu A., Lehmann, Paul V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3901103/
https://www.ncbi.nlm.nih.gov/pubmed/24710483
http://dx.doi.org/10.3390/cells1030409

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3901103/
https://www.ncbi.nlm.nih.gov/pubmed/24710483
http://dx.doi.org/10.3390/cells1030409

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