Ensuring good quality rna for quantitative real-time pcr isolated from renal proximal tubular cells using laser capture microdissection

BACKGROUND: In order to provide gene expression profiles of different cell types, the primary step is to isolate the specific cells of interest via laser capture microdissection (LCM), followed by extraction of good quality total RNA sufficient for quantitative real-time polymerase chain reaction (q...

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Detalles Bibliográficos
Autores principales: Yee, Jie Yin, Limenta, Lie Michael George, Rogers, Keith, Rogers, Susan Mary, Tay, Vanessa SY, Lee, Edmund JD
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3905289/
https://www.ncbi.nlm.nih.gov/pubmed/24467986
http://dx.doi.org/10.1186/1756-0500-7-62

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3905289/
https://www.ncbi.nlm.nih.gov/pubmed/24467986
http://dx.doi.org/10.1186/1756-0500-7-62

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