Cargando…

Ensuring good quality rna for quantitative real-time pcr isolated from renal proximal tubular cells using laser capture microdissection

BACKGROUND: In order to provide gene expression profiles of different cell types, the primary step is to isolate the specific cells of interest via laser capture microdissection (LCM), followed by extraction of good quality total RNA sufficient for quantitative real-time polymerase chain reaction (q...

Descripción completa

Detalles Bibliográficos
Autores principales:	Yee, Jie Yin, Limenta, Lie Michael George, Rogers, Keith, Rogers, Susan Mary, Tay, Vanessa SY, Lee, Edmund JD
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2014
Materias:	Technical Note
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3905289/ https://www.ncbi.nlm.nih.gov/pubmed/24467986 http://dx.doi.org/10.1186/1756-0500-7-62

Ejemplares similares

Laser Capture Microdissection and Multiplex-Tandem PCR Analysis of Proximal Tubular Epithelial Cell Signaling in Human Kidney Disease
por: Wilkinson, Ray, et al.
Publicado: (2014)

Comparison of RNA extraction kits and histological stains for laser capture microdissected prostate tissue
por: Kolijn, Kimberley, et al.
Publicado: (2016)

The PFA-AMeX method achieves a good balance between the morphology of tissues and the quality of RNA content in DNA microarray analysis with laser-capture microdissection samples
por: Watanabe, Takeshi, et al.
Publicado: (2014)

DNA Profiling of Spermatozoa by Laser Capture Microdissection and Low Volume-PCR
por: Li, Cai-xia, et al.
Publicado: (2011)

Laser Capture Microdissection in Dentistry
por: Chokechanachaisakul, Uraiwan, et al.
Publicado: (2010)

PAXgene-fixed paraffin-embedded sample is applicable to laser capture microdissection with well-balanced RNA quality and tissue morphology
por: Yamazaki, Masaki, et al.
Publicado: (2018)

Application of NeuroTrace staining in the fresh frozen brain samples to laser microdissection combined with quantitative RT-PCR analysis
por: Benner, Seico, et al.
Publicado: (2015)

Laser capture microdissection (LCM) and whole genome amplification (WGA) of DNA from normal breast tissue --- optimization for genome wide array analyses
por: Aaltonen, Kristina E, et al.
Publicado: (2011)

Shape decomposition algorithms for laser capture microdissection
por: Selbach, Leonie, et al.
Publicado: (2021)

Laser capture microdissection in forensic research: a review
por: Vandewoestyne, Mado, et al.
Publicado: (2010)

Laser capture microdissection of gonads from juvenile zebrafish
por: Jørgensen, Anne, et al.
Publicado: (2009)

The invasive proteome of glioblastoma revealed by laser-capture microdissection
por: Daubon, Thomas, et al.
Publicado: (2019)

Validation of Laser Capture Microdissection Protocol in Endometriosis Studies
por: da Costa, Katiane de Almeida, et al.
Publicado: (2019)

Implementation and evaluation of amyloidosis subtyping by laser-capture microdissection and tandem mass spectrometry
por: Mollee, Peter, et al.
Publicado: (2016)

The Cerebral Microvasculature in Schizophrenia: A Laser Capture Microdissection Study
por: Harris, Laura W., et al.
Publicado: (2008)

Laser Capture Microdissection Protocol for Xylem Tissues of Woody Plants
por: Blokhina, Olga, et al.
Publicado: (2017)

Laser capture microdissection for transcriptomic profiles in human skin biopsies
por: Santoro, Silvia, et al.
Publicado: (2018)

Identification of Inner Mucus-Associated Bacteria by Laser Capture Microdissection
por: Chassaing, Benoit, et al.
Publicado: (2018)

Laser Capture Microdissection: A Gear for Pancreatic Cancer Research
por: Rao, Bhavana Hemantha, et al.
Publicado: (2022)

Laser Microdissection of Specific Stem-Base Tissue Types from Olive Microcuttings for Isolation of High-Quality RNA
por: Velada, Isabel, et al.
Publicado: (2021)

Proteome analysis of human gastric cardia adenocarcinoma by laser capture microdissection
por: Cheng, Yan, et al.
Publicado: (2007)

Laser capture microdissection: from its principle to applications in research on neurodegeneration
por: Chung, Sook Hyun, et al.
Publicado: (2015)

A high-efficiency trichome collection system by laser capture microdissection
por: Qin, Wei, et al.
Publicado: (2022)

Pathway-Focused PCR Array Profiling of Enriched Populations of Laser Capture Microdissected Hippocampal Cells after Traumatic Brain Injury
por: Boone, Deborah R., et al.
Publicado: (2015)

Application of laser capture microdissection and PCR sequencing in the diagnosis of Coccidioides spp. infection: A case report and literature review in China
por: Yang, Xinyu, et al.
Publicado: (2021)

Microdissection: A method developed to investigate mechanisms involved in transmissible spongiform encephalopathy pathogenesis
por: Barr, Janice B, et al.
Publicado: (2004)

Suspension fluorescence in situ hybridization (S-FISH) combined with automatic detection and laser microdissection for STR profiling of male cells in male/female mixtures
por: Vandewoestyne, Mado, et al.
Publicado: (2009)

Laser capture microdissection enables cellular and molecular studies of tooth root development
por: Sun, Jian-Xun, et al.
Publicado: (2012)

Characterization of Glomerular Diseases Using Proteomic Analysis of Laser Capture Microdissected Glomeruli
por: Satoskar, AA, et al.
Publicado: (2012)

Proteomics Pipeline for Biomarker Discovery of Laser Capture Microdissected Breast Cancer Tissue
por: Liu, Ning Qing, et al.
Publicado: (2012)

Quantitative Proteomics in Laser Capture Microdissected Sleep Nuclei From Rat Brain
por: Miller, Ronald A., et al.
Publicado: (2014)

Fluorescence-based cell-specific detection for laser-capture microdissection in human brain
por: Rocco, Brad R., et al.
Publicado: (2017)

Efficacy of laser capture microdissection plus RT-PCR technique in analyzing gene expression levels in human gastric cancer and colon cancer
por: Makino, Hiroshi, et al.
Publicado: (2008)

Laser Capture Microdissection Revisited as a Tool for Transcriptomic Analysis: Application of an Excel-Based qPCR Preparation Software (PREXCEL-Q)
por: Sow, Fatoumata B., et al.
Publicado: (2009)

Optimization of laser capture microdissection and RNA amplification for gene expression profiling of prostate cancer
por: Kube, Dagmar M, et al.
Publicado: (2007)

SIVQ-aided laser capture microdissection: A tool for high-throughput expression profiling
por: Hipp, Jason, et al.
Publicado: (2011)

Creation of differentiation-specific genomic maps of human epidermis through laser capture microdissection
por: Gulati, Nicholas, et al.
Publicado: (2013)

Laser capture microdissection in Ectocarpus siliculosus: the pathway to cell-specific transcriptomics in brown algae
por: Saint-Marcoux, Denis, et al.
Publicado: (2015)

Stereotactic Atlas-Guided Laser Capture Microdissection of Brain Regions Affected by Traumatic Injury
por: Weisz, Harris A., et al.
Publicado: (2017)

A novel Microproteomic Approach Using Laser Capture Microdissection to Study Cellular Protrusions
por: Gousset, Karine, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_2bg915kcspe00jmvrt2q4ktkp5