Positive and negative selection using the tetA-sacB cassette: recombineering and P1 transduction in Escherichia coli

The two-step process of selection and counter-selection is a standard way to enable genetic modification and engineering of bacterial genomes using homologous recombination methods. The tetA and sacB genes are contained in a DNA cassette and confer a novel dual counter-selection system. Expression o...

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Detalles Bibliográficos
Autores principales: Li, Xin-tian, Thomason, Lynn C., Sawitzke, James A., Costantino, Nina, Court, Donald L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3905872/
https://www.ncbi.nlm.nih.gov/pubmed/24203710
http://dx.doi.org/10.1093/nar/gkt1075

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3905872/
https://www.ncbi.nlm.nih.gov/pubmed/24203710
http://dx.doi.org/10.1093/nar/gkt1075

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