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Positive and negative selection using the tetA-sacB cassette: recombineering and P1 transduction in Escherichia coli
The two-step process of selection and counter-selection is a standard way to enable genetic modification and engineering of bacterial genomes using homologous recombination methods. The tetA and sacB genes are contained in a DNA cassette and confer a novel dual counter-selection system. Expression o...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3905872/ https://www.ncbi.nlm.nih.gov/pubmed/24203710 http://dx.doi.org/10.1093/nar/gkt1075 |