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In-cell optical imaging of exogenous G-quadruplex DNA by fluorogenic ligands

Guanine-rich oligonucleotides (GROs) are promising therapeutic candidate for cancer treatment and other biomedical application. We have introduced a G-quadruplex (G4) ligand, 3,6-bis(1-methyl-4-vinylpyridinium) carbazole diiodide, to monitor the cellular uptake of naked GROs and map their intracellu...

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Autores principales: Tseng, Ting-Yuan, Wang, Zi-Fu, Chien, Cheng-Hao, Chang, Ta-Chau
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3905880/
https://www.ncbi.nlm.nih.gov/pubmed/24030712
http://dx.doi.org/10.1093/nar/gkt814
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author Tseng, Ting-Yuan
Wang, Zi-Fu
Chien, Cheng-Hao
Chang, Ta-Chau
author_facet Tseng, Ting-Yuan
Wang, Zi-Fu
Chien, Cheng-Hao
Chang, Ta-Chau
author_sort Tseng, Ting-Yuan
collection PubMed
description Guanine-rich oligonucleotides (GROs) are promising therapeutic candidate for cancer treatment and other biomedical application. We have introduced a G-quadruplex (G4) ligand, 3,6-bis(1-methyl-4-vinylpyridinium) carbazole diiodide, to monitor the cellular uptake of naked GROs and map their intracellular localizations in living cells by using confocal microscopy. The GROs that form parallel G4 structures, such as PU22, T40214 and AS1411, are detected mainly in the lysosome of CL1-0 lung cancer cells after incubation for 2 h. On the contrary, the GROs that form non-parallel G4 structures, such as human telomeres (HT23) and thrombin binding aptamer (TBA), are rarely detected in the lysosome, but found mainly in the mitochondria. Moreover, the fluorescence resonant energy transfer studies of fluorophore-labeled GROs show that the parallel G4 structures can be retained in CL1-0 cells, whereas the non-parallel G4 structures are likely distorted in CL1-0 cells after cellular uptake. Of interest is that the distorted G4 structure of HT23 from the non-parallel G4 structure can reform to a probable parallel G4 structure induced by a G4 ligand in CL1-0 living cells. These findings are valuable to the design and rationale behind the possible targeted drug delivery to specific cellular organelles using GROs.
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spelling pubmed-39058802014-01-29 In-cell optical imaging of exogenous G-quadruplex DNA by fluorogenic ligands Tseng, Ting-Yuan Wang, Zi-Fu Chien, Cheng-Hao Chang, Ta-Chau Nucleic Acids Res Structural Biology Guanine-rich oligonucleotides (GROs) are promising therapeutic candidate for cancer treatment and other biomedical application. We have introduced a G-quadruplex (G4) ligand, 3,6-bis(1-methyl-4-vinylpyridinium) carbazole diiodide, to monitor the cellular uptake of naked GROs and map their intracellular localizations in living cells by using confocal microscopy. The GROs that form parallel G4 structures, such as PU22, T40214 and AS1411, are detected mainly in the lysosome of CL1-0 lung cancer cells after incubation for 2 h. On the contrary, the GROs that form non-parallel G4 structures, such as human telomeres (HT23) and thrombin binding aptamer (TBA), are rarely detected in the lysosome, but found mainly in the mitochondria. Moreover, the fluorescence resonant energy transfer studies of fluorophore-labeled GROs show that the parallel G4 structures can be retained in CL1-0 cells, whereas the non-parallel G4 structures are likely distorted in CL1-0 cells after cellular uptake. Of interest is that the distorted G4 structure of HT23 from the non-parallel G4 structure can reform to a probable parallel G4 structure induced by a G4 ligand in CL1-0 living cells. These findings are valuable to the design and rationale behind the possible targeted drug delivery to specific cellular organelles using GROs. Oxford University Press 2013-12 2013-09-11 /pmc/articles/PMC3905880/ /pubmed/24030712 http://dx.doi.org/10.1093/nar/gkt814 Text en © The Author(s) 2013. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Structural Biology
Tseng, Ting-Yuan
Wang, Zi-Fu
Chien, Cheng-Hao
Chang, Ta-Chau
In-cell optical imaging of exogenous G-quadruplex DNA by fluorogenic ligands
title In-cell optical imaging of exogenous G-quadruplex DNA by fluorogenic ligands
title_full In-cell optical imaging of exogenous G-quadruplex DNA by fluorogenic ligands
title_fullStr In-cell optical imaging of exogenous G-quadruplex DNA by fluorogenic ligands
title_full_unstemmed In-cell optical imaging of exogenous G-quadruplex DNA by fluorogenic ligands
title_short In-cell optical imaging of exogenous G-quadruplex DNA by fluorogenic ligands
title_sort in-cell optical imaging of exogenous g-quadruplex dna by fluorogenic ligands
topic Structural Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3905880/
https://www.ncbi.nlm.nih.gov/pubmed/24030712
http://dx.doi.org/10.1093/nar/gkt814
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