Ancestral mutations as a tool for solubilizing proteins: The case of a hydrophobic phosphate-binding protein

Stable and soluble proteins are ideal candidates for functional and structural studies. Unfortunately, some proteins or enzymes can be difficult to isolate, being sometimes poorly expressed in heterologous systems, insoluble and/or unstable. Numerous methods have been developed to address these issu...

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Detalles Bibliográficos
Autores principales: Gonzalez, Daniel, Hiblot, Julien, Darbinian, Nune, Miller, Jernelle C., Gotthard, Guillaume, Amini, Shohreh, Chabriere, Eric, Elias, Mikael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3907688/
https://www.ncbi.nlm.nih.gov/pubmed/24490136
http://dx.doi.org/10.1016/j.fob.2013.12.006

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3907688/
https://www.ncbi.nlm.nih.gov/pubmed/24490136
http://dx.doi.org/10.1016/j.fob.2013.12.006

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