High-Level Expression of Pro-Form Lipase from Rhizopus oryzae in Pichia pastoris and Its Purification and Characterization

A gene encoding Rhizopus oryzae lipase containing prosequence (ProROL) was cloned into the pPICZαA and electrotransformed into the Pichia pastoris X-33 strain. The lipase was functionally expressed and secreted in Pichia pastoris with a molecular weight of 35 kDa. The maximum lipase activity of reco...

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Detalles Bibliográficos
Autores principales: Wang, Jian-Rong, Li, Yang-Yuan, Xu, Shu-De, Li, Peng, Liu, Jing-Shan, Liu, Dan-Ni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3907806/
https://www.ncbi.nlm.nih.gov/pubmed/24368519
http://dx.doi.org/10.3390/ijms15010203

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3907806/
https://www.ncbi.nlm.nih.gov/pubmed/24368519
http://dx.doi.org/10.3390/ijms15010203

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