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High-Level Expression of Pro-Form Lipase from Rhizopus oryzae in Pichia pastoris and Its Purification and Characterization
A gene encoding Rhizopus oryzae lipase containing prosequence (ProROL) was cloned into the pPICZαA and electrotransformed into the Pichia pastoris X-33 strain. The lipase was functionally expressed and secreted in Pichia pastoris with a molecular weight of 35 kDa. The maximum lipase activity of reco...
Autores principales: | Wang, Jian-Rong, Li, Yang-Yuan, Xu, Shu-De, Li, Peng, Liu, Jing-Shan, Liu, Dan-Ni |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3907806/ https://www.ncbi.nlm.nih.gov/pubmed/24368519 http://dx.doi.org/10.3390/ijms15010203 |
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