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Development and Experimental Testing of an Optical Micro-Spectroscopic Technique Incorporating True Line-Scan Excitation

Multiphoton micro-spectroscopy, employing diffraction optics and electron-multiplying CCD (EMCCD) cameras, is a suitable method for determining protein complex stoichiometry, quaternary structure, and spatial distribution in living cells using Förster resonance energy transfer (FRET) imaging. The me...

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Detalles Bibliográficos
Autores principales: Biener, Gabriel, Stoneman, Michael R., Acbas, Gheorghe, Holz, Jessica D., Orlova, Marianna, Komarova, Liudmila, Kuchin, Sergei, Raicu, Valerică
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3907809/
https://www.ncbi.nlm.nih.gov/pubmed/24378851
http://dx.doi.org/10.3390/ijms15010261