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Specific and Reversible Immobilization of Proteins Tagged to the Affinity Polypeptide C-LytA on Functionalized Graphite Electrodes

We have developed a general method for the specific and reversible immobilization of proteins fused to the choline-binding module C-LytA on functionalized graphite electrodes. Graphite electrode surfaces were modified by diazonium chemistry to introduce carboxylic groups that were subsequently used...

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Detalles Bibliográficos
Autores principales: Bello-Gil, Daniel, Maestro, Beatriz, Fonseca, Jennifer, Feliu, Juan M., Climent, Víctor, Sanz, Jesús M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3909327/
https://www.ncbi.nlm.nih.gov/pubmed/24498237
http://dx.doi.org/10.1371/journal.pone.0087995