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Specific and Reversible Immobilization of Proteins Tagged to the Affinity Polypeptide C-LytA on Functionalized Graphite Electrodes
We have developed a general method for the specific and reversible immobilization of proteins fused to the choline-binding module C-LytA on functionalized graphite electrodes. Graphite electrode surfaces were modified by diazonium chemistry to introduce carboxylic groups that were subsequently used...
Autores principales: | Bello-Gil, Daniel, Maestro, Beatriz, Fonseca, Jennifer, Feliu, Juan M., Climent, Víctor, Sanz, Jesús M. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3909327/ https://www.ncbi.nlm.nih.gov/pubmed/24498237 http://dx.doi.org/10.1371/journal.pone.0087995 |
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