A Recombinant Rabies Virus Encoding Two Copies of the Glycoprotein Gene Confers Protection in Dogs against a Virulent Challenge

The rabies virus (RABV) glycoprotein (G) is the principal antigen responsible for the induction of virus neutralizing antibodies (VNA) and is the major modality of protective immunity in animals. A recombinant RABV HEP-Flury strain was generated by reverse genetics to encode two copies of the G-gene...

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Autores principales: Liu, Xiaohui, Yang, Youtian, Sun, Zhaojin, Chen, Jing, Ai, Jun, Dun, Can, Fu, Zhen F., Niu, Xuefeng, Guo, Xiaofeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3911940/
https://www.ncbi.nlm.nih.gov/pubmed/24498294
http://dx.doi.org/10.1371/journal.pone.0087105
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author Liu, Xiaohui
Yang, Youtian
Sun, Zhaojin
Chen, Jing
Ai, Jun
Dun, Can
Fu, Zhen F.
Niu, Xuefeng
Guo, Xiaofeng
author_facet Liu, Xiaohui
Yang, Youtian
Sun, Zhaojin
Chen, Jing
Ai, Jun
Dun, Can
Fu, Zhen F.
Niu, Xuefeng
Guo, Xiaofeng
author_sort Liu, Xiaohui
collection PubMed
description The rabies virus (RABV) glycoprotein (G) is the principal antigen responsible for the induction of virus neutralizing antibodies (VNA) and is the major modality of protective immunity in animals. A recombinant RABV HEP-Flury strain was generated by reverse genetics to encode two copies of the G-gene (referred to as HEP-dG). The biological properties of HEP-dG were compared to those of the parental virus (HEP-Flury strain). The HEP-dG recombinant virus grew 100 times more efficiently in BHK-21 cell than the parental virus, yet the virulence of the dG recombinant virus in suckling mice was lower than the parental virus. The HEP-dG virus can improve the expression of G-gene mRNA and the G protein and produce more offspring viruses in cells. The amount of G protein revealed a positive relationship with immunogenicity in mice and dogs. The inactivated HEP-dG recombinant virus induced higher levels of VNA and conferred better protection against virulent RABV in mice and dogs than the inactivated parental virus and a commercial vaccine. The protective antibody persisted for at least 12 months. These data demonstrate that the HEP-dG is stable, induces a strong VNA response and confers protective immunity more effectively than the RABV HEP-Flury strain. HEP-dG could be a potential candidate in the development of novel inactivated rabies vaccines
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spelling pubmed-39119402014-02-04 A Recombinant Rabies Virus Encoding Two Copies of the Glycoprotein Gene Confers Protection in Dogs against a Virulent Challenge Liu, Xiaohui Yang, Youtian Sun, Zhaojin Chen, Jing Ai, Jun Dun, Can Fu, Zhen F. Niu, Xuefeng Guo, Xiaofeng PLoS One Research Article The rabies virus (RABV) glycoprotein (G) is the principal antigen responsible for the induction of virus neutralizing antibodies (VNA) and is the major modality of protective immunity in animals. A recombinant RABV HEP-Flury strain was generated by reverse genetics to encode two copies of the G-gene (referred to as HEP-dG). The biological properties of HEP-dG were compared to those of the parental virus (HEP-Flury strain). The HEP-dG recombinant virus grew 100 times more efficiently in BHK-21 cell than the parental virus, yet the virulence of the dG recombinant virus in suckling mice was lower than the parental virus. The HEP-dG virus can improve the expression of G-gene mRNA and the G protein and produce more offspring viruses in cells. The amount of G protein revealed a positive relationship with immunogenicity in mice and dogs. The inactivated HEP-dG recombinant virus induced higher levels of VNA and conferred better protection against virulent RABV in mice and dogs than the inactivated parental virus and a commercial vaccine. The protective antibody persisted for at least 12 months. These data demonstrate that the HEP-dG is stable, induces a strong VNA response and confers protective immunity more effectively than the RABV HEP-Flury strain. HEP-dG could be a potential candidate in the development of novel inactivated rabies vaccines Public Library of Science 2014-02-03 /pmc/articles/PMC3911940/ /pubmed/24498294 http://dx.doi.org/10.1371/journal.pone.0087105 Text en © 2014 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Liu, Xiaohui
Yang, Youtian
Sun, Zhaojin
Chen, Jing
Ai, Jun
Dun, Can
Fu, Zhen F.
Niu, Xuefeng
Guo, Xiaofeng
A Recombinant Rabies Virus Encoding Two Copies of the Glycoprotein Gene Confers Protection in Dogs against a Virulent Challenge
title A Recombinant Rabies Virus Encoding Two Copies of the Glycoprotein Gene Confers Protection in Dogs against a Virulent Challenge
title_full A Recombinant Rabies Virus Encoding Two Copies of the Glycoprotein Gene Confers Protection in Dogs against a Virulent Challenge
title_fullStr A Recombinant Rabies Virus Encoding Two Copies of the Glycoprotein Gene Confers Protection in Dogs against a Virulent Challenge
title_full_unstemmed A Recombinant Rabies Virus Encoding Two Copies of the Glycoprotein Gene Confers Protection in Dogs against a Virulent Challenge
title_short A Recombinant Rabies Virus Encoding Two Copies of the Glycoprotein Gene Confers Protection in Dogs against a Virulent Challenge
title_sort recombinant rabies virus encoding two copies of the glycoprotein gene confers protection in dogs against a virulent challenge
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3911940/
https://www.ncbi.nlm.nih.gov/pubmed/24498294
http://dx.doi.org/10.1371/journal.pone.0087105
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