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Hes1 is involved in the self-renewal and tumourigenicity of stem-like cancer cells in colon cancer
A small subpopulation of cancer cells with stem cell-like features might be responsible for tumour generation, progression, and chemoresistance. Hes1 influences the maintenance of certain stem cells and progenitor cells and the digestive systems. We found upregulated Hes1 in poorly differentiated ca...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3912485/ https://www.ncbi.nlm.nih.gov/pubmed/24492635 http://dx.doi.org/10.1038/srep03963 |
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author | Gao, Fei Zhang, YuQin Wang, ShengChun Liu, YuanQiang Zheng, Lin Yang, JianQuan Huang, Wei Ye, YanFen Luo, WeiRen Xiao, Dong |
author_facet | Gao, Fei Zhang, YuQin Wang, ShengChun Liu, YuanQiang Zheng, Lin Yang, JianQuan Huang, Wei Ye, YanFen Luo, WeiRen Xiao, Dong |
author_sort | Gao, Fei |
collection | PubMed |
description | A small subpopulation of cancer cells with stem cell-like features might be responsible for tumour generation, progression, and chemoresistance. Hes1 influences the maintenance of certain stem cells and progenitor cells and the digestive systems. We found upregulated Hes1 in poorly differentiated cancer samples compared with well-differentiated tumour samples, and most of the adenocarcinomas exhibited significantly higher levels of Hes1 mRNA compared with that observed in matched normal colon samples. Moreover, Hes1 expression was found to be correlated with the expression of stem cell markers in colon cancer samples, and Hes1 upregulates the expression of stemness-related genes in colon cancer cells. In addition, Hes1 enhances the self-renewal properties of the stem-like cells by increasing the sizes of CD133+ cells and SP cells and the ability of tumour sphere formation. Additionally, the Hes1-overexpressing cells formed significantly larger and higher number of colonies, as determined through the colony and the soft agar assays. More importantly, Hes1 enhances the tumourigenicity of colon cancer cell lines in nude mice and exhibits a strong tumour-formation ability at a cell density of 1 × 10(3). Taken together, our data indicate that Hes1 induces stem-like cell self-renewal and increases the number of tumour-initiating cells in colon cancer. |
format | Online Article Text |
id | pubmed-3912485 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-39124852014-02-04 Hes1 is involved in the self-renewal and tumourigenicity of stem-like cancer cells in colon cancer Gao, Fei Zhang, YuQin Wang, ShengChun Liu, YuanQiang Zheng, Lin Yang, JianQuan Huang, Wei Ye, YanFen Luo, WeiRen Xiao, Dong Sci Rep Article A small subpopulation of cancer cells with stem cell-like features might be responsible for tumour generation, progression, and chemoresistance. Hes1 influences the maintenance of certain stem cells and progenitor cells and the digestive systems. We found upregulated Hes1 in poorly differentiated cancer samples compared with well-differentiated tumour samples, and most of the adenocarcinomas exhibited significantly higher levels of Hes1 mRNA compared with that observed in matched normal colon samples. Moreover, Hes1 expression was found to be correlated with the expression of stem cell markers in colon cancer samples, and Hes1 upregulates the expression of stemness-related genes in colon cancer cells. In addition, Hes1 enhances the self-renewal properties of the stem-like cells by increasing the sizes of CD133+ cells and SP cells and the ability of tumour sphere formation. Additionally, the Hes1-overexpressing cells formed significantly larger and higher number of colonies, as determined through the colony and the soft agar assays. More importantly, Hes1 enhances the tumourigenicity of colon cancer cell lines in nude mice and exhibits a strong tumour-formation ability at a cell density of 1 × 10(3). Taken together, our data indicate that Hes1 induces stem-like cell self-renewal and increases the number of tumour-initiating cells in colon cancer. Nature Publishing Group 2014-02-04 /pmc/articles/PMC3912485/ /pubmed/24492635 http://dx.doi.org/10.1038/srep03963 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Article Gao, Fei Zhang, YuQin Wang, ShengChun Liu, YuanQiang Zheng, Lin Yang, JianQuan Huang, Wei Ye, YanFen Luo, WeiRen Xiao, Dong Hes1 is involved in the self-renewal and tumourigenicity of stem-like cancer cells in colon cancer |
title | Hes1 is involved in the self-renewal and tumourigenicity of stem-like cancer cells in colon cancer |
title_full | Hes1 is involved in the self-renewal and tumourigenicity of stem-like cancer cells in colon cancer |
title_fullStr | Hes1 is involved in the self-renewal and tumourigenicity of stem-like cancer cells in colon cancer |
title_full_unstemmed | Hes1 is involved in the self-renewal and tumourigenicity of stem-like cancer cells in colon cancer |
title_short | Hes1 is involved in the self-renewal and tumourigenicity of stem-like cancer cells in colon cancer |
title_sort | hes1 is involved in the self-renewal and tumourigenicity of stem-like cancer cells in colon cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3912485/ https://www.ncbi.nlm.nih.gov/pubmed/24492635 http://dx.doi.org/10.1038/srep03963 |
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