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Neprilysin Is Poorly Expressed in the Prefrontal Cortex of Aged Dogs with Cognitive Dysfunction Syndrome

Neprilysin (NEP) is the principal amyloid β (Aβ) degrading peptidase; this activity may protect against Alzheimer's disease (AD), the most important age-related neurodegenerative process. The aim of this work was to analyze NEP mRNA expression in the frontal cortex of dogs with and without cani...

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Autores principales: Canudas, Jesús, Insua, Daniel, Sarasa, Leticia, González-Martínez, Ángela, Suárez, María Luisa, Santamarina, Germán, Pesini, Pedro, Sarasa, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3912887/
https://www.ncbi.nlm.nih.gov/pubmed/24511411
http://dx.doi.org/10.1155/2014/483281
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author Canudas, Jesús
Insua, Daniel
Sarasa, Leticia
González-Martínez, Ángela
Suárez, María Luisa
Santamarina, Germán
Pesini, Pedro
Sarasa, Manuel
author_facet Canudas, Jesús
Insua, Daniel
Sarasa, Leticia
González-Martínez, Ángela
Suárez, María Luisa
Santamarina, Germán
Pesini, Pedro
Sarasa, Manuel
author_sort Canudas, Jesús
collection PubMed
description Neprilysin (NEP) is the principal amyloid β (Aβ) degrading peptidase; this activity may protect against Alzheimer's disease (AD), the most important age-related neurodegenerative process. The aim of this work was to analyze NEP mRNA expression in the frontal cortex of dogs with and without canine cognitive dysfunction syndrome (CDS), which is considered a natural model for AD. Expression of canine cerebral NEP mRNA was assessed by RT-PCR followed by qPCR in young, aged-cognitively unimpaired (CU), and aged-cognitively impaired (CI) dogs. On average, aged-CI dogs showed 80% (P < 0.01) lower expression levels of NEP mRNA than their aged-CU counterparts. Furthermore, the standard deviation of the qPCR measurements was more than 6 times higher in the cognitively healthy animals (young and aged-CU) than in the aged-CI group. Another interesting find is the determination of a positive correlation between NEP expression and the number of cholinergic neurons in basal telencephalon, indicating a probable connection between both events in these types of neurodegeneration processes. These results suggest that high expression levels of NEP might be a protective factor for canine CDS and, most likely, for other Aβ-associated neurodegenerative diseases, such as AD.
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spelling pubmed-39128872014-02-09 Neprilysin Is Poorly Expressed in the Prefrontal Cortex of Aged Dogs with Cognitive Dysfunction Syndrome Canudas, Jesús Insua, Daniel Sarasa, Leticia González-Martínez, Ángela Suárez, María Luisa Santamarina, Germán Pesini, Pedro Sarasa, Manuel Int J Alzheimers Dis Research Article Neprilysin (NEP) is the principal amyloid β (Aβ) degrading peptidase; this activity may protect against Alzheimer's disease (AD), the most important age-related neurodegenerative process. The aim of this work was to analyze NEP mRNA expression in the frontal cortex of dogs with and without canine cognitive dysfunction syndrome (CDS), which is considered a natural model for AD. Expression of canine cerebral NEP mRNA was assessed by RT-PCR followed by qPCR in young, aged-cognitively unimpaired (CU), and aged-cognitively impaired (CI) dogs. On average, aged-CI dogs showed 80% (P < 0.01) lower expression levels of NEP mRNA than their aged-CU counterparts. Furthermore, the standard deviation of the qPCR measurements was more than 6 times higher in the cognitively healthy animals (young and aged-CU) than in the aged-CI group. Another interesting find is the determination of a positive correlation between NEP expression and the number of cholinergic neurons in basal telencephalon, indicating a probable connection between both events in these types of neurodegeneration processes. These results suggest that high expression levels of NEP might be a protective factor for canine CDS and, most likely, for other Aβ-associated neurodegenerative diseases, such as AD. Hindawi Publishing Corporation 2014 2014-01-06 /pmc/articles/PMC3912887/ /pubmed/24511411 http://dx.doi.org/10.1155/2014/483281 Text en Copyright © 2014 Jesús Canudas et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Canudas, Jesús
Insua, Daniel
Sarasa, Leticia
González-Martínez, Ángela
Suárez, María Luisa
Santamarina, Germán
Pesini, Pedro
Sarasa, Manuel
Neprilysin Is Poorly Expressed in the Prefrontal Cortex of Aged Dogs with Cognitive Dysfunction Syndrome
title Neprilysin Is Poorly Expressed in the Prefrontal Cortex of Aged Dogs with Cognitive Dysfunction Syndrome
title_full Neprilysin Is Poorly Expressed in the Prefrontal Cortex of Aged Dogs with Cognitive Dysfunction Syndrome
title_fullStr Neprilysin Is Poorly Expressed in the Prefrontal Cortex of Aged Dogs with Cognitive Dysfunction Syndrome
title_full_unstemmed Neprilysin Is Poorly Expressed in the Prefrontal Cortex of Aged Dogs with Cognitive Dysfunction Syndrome
title_short Neprilysin Is Poorly Expressed in the Prefrontal Cortex of Aged Dogs with Cognitive Dysfunction Syndrome
title_sort neprilysin is poorly expressed in the prefrontal cortex of aged dogs with cognitive dysfunction syndrome
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3912887/
https://www.ncbi.nlm.nih.gov/pubmed/24511411
http://dx.doi.org/10.1155/2014/483281
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