High-throughput strategies for penicillin G acylase production in rE. coli fed-batch cultivations

BACKGROUND: Penicillin G acylase (PGA) is used industrially to catalyze the hydrolysis of penicillin G to obtain 6-aminopenicillanic acid. In Escherichia coli, the most-studied microorganism for PGA production, this enzyme accumulates in the periplasmic cell space, and temperature plays an important...

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Detalles Bibliográficos
Autores principales: Vélez, Ana Maria, da Silva, Adilson José, Luperni Horta, Antonio Carlos, Sargo, Cintia Regina, Campani, Gilson, Gonçalves Silva, Gabriel, de Lima Camargo Giordano, Raquel, Zangirolami, Teresa Cristina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3913322/
https://www.ncbi.nlm.nih.gov/pubmed/24444109
http://dx.doi.org/10.1186/1472-6750-14-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3913322/
https://www.ncbi.nlm.nih.gov/pubmed/24444109
http://dx.doi.org/10.1186/1472-6750-14-6

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