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Destabilized eYFP variants for dynamic gene expression studies in Corynebacterium glutamicum
Fluorescent reporter proteins are widely used for the non-invasive monitoring of gene expression patterns, but dynamic measurements are hampered by the extremely high stability of GFP and homologue proteins. In this study, we used SsrA-mediated peptide tagging for the construction of unstable varian...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3917462/ https://www.ncbi.nlm.nih.gov/pubmed/22938655 http://dx.doi.org/10.1111/j.1751-7915.2012.00360.x |