Destabilized eYFP variants for dynamic gene expression studies in Corynebacterium glutamicum

Fluorescent reporter proteins are widely used for the non-invasive monitoring of gene expression patterns, but dynamic measurements are hampered by the extremely high stability of GFP and homologue proteins. In this study, we used SsrA-mediated peptide tagging for the construction of unstable varian...

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Detalles Bibliográficos
Autores principales: Hentschel, Eva, Will, Cornelia, Mustafi, Nurije, Burkovski, Andreas, Rehm, Nadine, Frunzke, Julia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2013
Materias:
Brief Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3917462/
https://www.ncbi.nlm.nih.gov/pubmed/22938655
http://dx.doi.org/10.1111/j.1751-7915.2012.00360.x
Descripción
Sumario:Fluorescent reporter proteins are widely used for the non-invasive monitoring of gene expression patterns, but dynamic measurements are hampered by the extremely high stability of GFP and homologue proteins. In this study, we used SsrA-mediated peptide tagging for the construction of unstable variants of the GFP derivative eYFP (enhanced yellow fluorescent protein) and applied those for transient gene expression analysis in the industrial platform organism Corynebacterium glutamicum.

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