Destabilized eYFP variants for dynamic gene expression studies in Corynebacterium glutamicum

Fluorescent reporter proteins are widely used for the non-invasive monitoring of gene expression patterns, but dynamic measurements are hampered by the extremely high stability of GFP and homologue proteins. In this study, we used SsrA-mediated peptide tagging for the construction of unstable varian...

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Detalles Bibliográficos
Autores principales: Hentschel, Eva, Will, Cornelia, Mustafi, Nurije, Burkovski, Andreas, Rehm, Nadine, Frunzke, Julia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2013
Materias:
Brief Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3917462/
https://www.ncbi.nlm.nih.gov/pubmed/22938655
http://dx.doi.org/10.1111/j.1751-7915.2012.00360.x
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author Hentschel, Eva
Will, Cornelia
Mustafi, Nurije
Burkovski, Andreas
Rehm, Nadine
Frunzke, Julia
author_facet Hentschel, Eva
Will, Cornelia
Mustafi, Nurije
Burkovski, Andreas
Rehm, Nadine
Frunzke, Julia
author_sort Hentschel, Eva
collection PubMed
description Fluorescent reporter proteins are widely used for the non-invasive monitoring of gene expression patterns, but dynamic measurements are hampered by the extremely high stability of GFP and homologue proteins. In this study, we used SsrA-mediated peptide tagging for the construction of unstable variants of the GFP derivative eYFP (enhanced yellow fluorescent protein) and applied those for transient gene expression analysis in the industrial platform organism Corynebacterium glutamicum.
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spelling pubmed-39174622014-02-12 Destabilized eYFP variants for dynamic gene expression studies in Corynebacterium glutamicum Hentschel, Eva Will, Cornelia Mustafi, Nurije Burkovski, Andreas Rehm, Nadine Frunzke, Julia Microb Biotechnol Brief Reports Fluorescent reporter proteins are widely used for the non-invasive monitoring of gene expression patterns, but dynamic measurements are hampered by the extremely high stability of GFP and homologue proteins. In this study, we used SsrA-mediated peptide tagging for the construction of unstable variants of the GFP derivative eYFP (enhanced yellow fluorescent protein) and applied those for transient gene expression analysis in the industrial platform organism Corynebacterium glutamicum. Blackwell Publishing Ltd 2013-03 2012-09-03 /pmc/articles/PMC3917462/ /pubmed/22938655 http://dx.doi.org/10.1111/j.1751-7915.2012.00360.x Text en © 2012 The Authors Microbial Biotechnology © 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
spellingShingle Brief Reports
Hentschel, Eva
Will, Cornelia
Mustafi, Nurije
Burkovski, Andreas
Rehm, Nadine
Frunzke, Julia
Destabilized eYFP variants for dynamic gene expression studies in Corynebacterium glutamicum
title Destabilized eYFP variants for dynamic gene expression studies in Corynebacterium glutamicum
title_full Destabilized eYFP variants for dynamic gene expression studies in Corynebacterium glutamicum
title_fullStr Destabilized eYFP variants for dynamic gene expression studies in Corynebacterium glutamicum
title_full_unstemmed Destabilized eYFP variants for dynamic gene expression studies in Corynebacterium glutamicum
title_short Destabilized eYFP variants for dynamic gene expression studies in Corynebacterium glutamicum
title_sort destabilized eyfp variants for dynamic gene expression studies in corynebacterium glutamicum
topic Brief Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3917462/
https://www.ncbi.nlm.nih.gov/pubmed/22938655
http://dx.doi.org/10.1111/j.1751-7915.2012.00360.x
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