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Expression of single-chain variable fragments fused with the Fc-region of rabbit IgG in Leishmania tarentolae
BACKGROUND: In recent years the generation of antibodies by recombinant methods, such as phage display technology, has increased the speed by which antibodies can be obtained. However, in some cases when recombinant antibodies have to be validated, expression in E. coli can be problematic. This prim...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3917567/ https://www.ncbi.nlm.nih.gov/pubmed/24428896 http://dx.doi.org/10.1186/1475-2859-13-9 |