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Acute murine antigen-induced arthritis is not affected by disruption of osteoblastic glucocorticoid signalling

BACKGROUND: The role of endogenous glucocorticoids (GC) in the initiation and maintenance of rheumatoid arthritis (RA) remains unclear. We demonstrated previously that disruption of GC signalling in osteoblasts results in a profound attenuation of K/BxN serum-induced arthritis, a mouse model of RA....

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Autores principales: Spies, Cornelia M, Wiebe, Edgar, Tu, Jinwen, Li, Aiqing, Gaber, Timo, Huscher, Dörte, Seibel, Markus J, Zhou, Hong, Buttgereit, Frank
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3922092/
https://www.ncbi.nlm.nih.gov/pubmed/24491163
http://dx.doi.org/10.1186/1471-2474-15-31
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author Spies, Cornelia M
Wiebe, Edgar
Tu, Jinwen
Li, Aiqing
Gaber, Timo
Huscher, Dörte
Seibel, Markus J
Zhou, Hong
Buttgereit, Frank
author_facet Spies, Cornelia M
Wiebe, Edgar
Tu, Jinwen
Li, Aiqing
Gaber, Timo
Huscher, Dörte
Seibel, Markus J
Zhou, Hong
Buttgereit, Frank
author_sort Spies, Cornelia M
collection PubMed
description BACKGROUND: The role of endogenous glucocorticoids (GC) in the initiation and maintenance of rheumatoid arthritis (RA) remains unclear. We demonstrated previously that disruption of GC signalling in osteoblasts results in a profound attenuation of K/BxN serum-induced arthritis, a mouse model of RA. To determine whether or not the modulation of the inflammatory response by osteoblasts involves T cells, we studied the effects of disrupted osteoblastic GC-signalling in the T cell-dependent model of antigen-induced arthritis (AIA). METHODS: Acute arthritis was induced in pre-immunised 11-week-old male 11β-hydroxysteroid dehydrogenase type 2 transgenic (tg) mice and their wild-type (WT) littermates by intra-articular injection of methylated bovine serum albumine (mBSA) into one knee joint. Knee diameter was measured every 1–2 days until euthanasia on day 14 post injection. In a separate experiment, arthritis was maintained for 28 days by weekly reinjections of mBSA. Tissues were analysed by histology, histomorphometry and microfocal-computed tomography. Serum cytokines levels were determined by multiplex suspension array. RESULTS: In both short and long term experiments, arthritis developed in tg and WT mice with no significant difference between both groups. Histological indices of inflammation, cartilage damage and bone erosion were similar in tg and WT mice. Bone volume and turnover at the contralateral tibia and systemic cytokine levels were not different. CONCLUSIONS: Acute murine AIA is not affected by a disruption in osteoblastic GC signalling. These data indicate that osteoblasts do not modulate the T cell-mediated inflammatory response via a GC-dependent pathway.
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spelling pubmed-39220922014-02-13 Acute murine antigen-induced arthritis is not affected by disruption of osteoblastic glucocorticoid signalling Spies, Cornelia M Wiebe, Edgar Tu, Jinwen Li, Aiqing Gaber, Timo Huscher, Dörte Seibel, Markus J Zhou, Hong Buttgereit, Frank BMC Musculoskelet Disord Research Article BACKGROUND: The role of endogenous glucocorticoids (GC) in the initiation and maintenance of rheumatoid arthritis (RA) remains unclear. We demonstrated previously that disruption of GC signalling in osteoblasts results in a profound attenuation of K/BxN serum-induced arthritis, a mouse model of RA. To determine whether or not the modulation of the inflammatory response by osteoblasts involves T cells, we studied the effects of disrupted osteoblastic GC-signalling in the T cell-dependent model of antigen-induced arthritis (AIA). METHODS: Acute arthritis was induced in pre-immunised 11-week-old male 11β-hydroxysteroid dehydrogenase type 2 transgenic (tg) mice and their wild-type (WT) littermates by intra-articular injection of methylated bovine serum albumine (mBSA) into one knee joint. Knee diameter was measured every 1–2 days until euthanasia on day 14 post injection. In a separate experiment, arthritis was maintained for 28 days by weekly reinjections of mBSA. Tissues were analysed by histology, histomorphometry and microfocal-computed tomography. Serum cytokines levels were determined by multiplex suspension array. RESULTS: In both short and long term experiments, arthritis developed in tg and WT mice with no significant difference between both groups. Histological indices of inflammation, cartilage damage and bone erosion were similar in tg and WT mice. Bone volume and turnover at the contralateral tibia and systemic cytokine levels were not different. CONCLUSIONS: Acute murine AIA is not affected by a disruption in osteoblastic GC signalling. These data indicate that osteoblasts do not modulate the T cell-mediated inflammatory response via a GC-dependent pathway. BioMed Central 2014-02-03 /pmc/articles/PMC3922092/ /pubmed/24491163 http://dx.doi.org/10.1186/1471-2474-15-31 Text en Copyright © 2014 Spies et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Spies, Cornelia M
Wiebe, Edgar
Tu, Jinwen
Li, Aiqing
Gaber, Timo
Huscher, Dörte
Seibel, Markus J
Zhou, Hong
Buttgereit, Frank
Acute murine antigen-induced arthritis is not affected by disruption of osteoblastic glucocorticoid signalling
title Acute murine antigen-induced arthritis is not affected by disruption of osteoblastic glucocorticoid signalling
title_full Acute murine antigen-induced arthritis is not affected by disruption of osteoblastic glucocorticoid signalling
title_fullStr Acute murine antigen-induced arthritis is not affected by disruption of osteoblastic glucocorticoid signalling
title_full_unstemmed Acute murine antigen-induced arthritis is not affected by disruption of osteoblastic glucocorticoid signalling
title_short Acute murine antigen-induced arthritis is not affected by disruption of osteoblastic glucocorticoid signalling
title_sort acute murine antigen-induced arthritis is not affected by disruption of osteoblastic glucocorticoid signalling
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3922092/
https://www.ncbi.nlm.nih.gov/pubmed/24491163
http://dx.doi.org/10.1186/1471-2474-15-31
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