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Zip nucleic acid: a new reliable method to increase the melting temperature of real-time PCR probes
TaqMan genotyping with real-time PCR is a reliable method for single nucleotide polymorphism detection, which is done by probes. These oligonucleotides should be short enough to avoid mismatch hybridization, as well as having 5–10°C higher melting temperature than the primers of real-time PCR reacti...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2014
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3922743/ https://www.ncbi.nlm.nih.gov/pubmed/24495816 http://dx.doi.org/10.1186/2251-6581-13-26 |