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EMS mutagenesis in mature seed-derived rice calli as a new method for rapidly obtaining TILLING mutant populations

BACKGROUND: TILLING (Targeting Induced Local Lesions IN Genomes) is a reverse genetic method that combines chemical mutagenesis with high-throughput genome-wide screening for point mutation detection in genes of interest. However, this mutation discovery approach faces a particular problem which is...

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Detalles Bibliográficos
Autores principales: Serrat, Xavier, Esteban, Roger, Guibourt, Nathalie, Moysset, Luisa, Nogués, Salvador, Lalanne, Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923009/
https://www.ncbi.nlm.nih.gov/pubmed/24475756
http://dx.doi.org/10.1186/1746-4811-10-5