Mass spectrometry–based relative quantification of proteins in precatalytic and catalytically active spliceosomes by metabolic labeling (SILAC), chemical labeling (iTRAQ), and label-free spectral count

The spliceosome undergoes major changes in protein and RNA composition during pre-mRNA splicing. Knowing the proteins—and their respective quantities—at each spliceosomal assembly stage is critical for understanding the molecular mechanisms and regulation of splicing. Here, we applied three independ...

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Detalles Bibliográficos
Autores principales: Schmidt, Carla, Grønborg, Mads, Deckert, Jochen, Bessonov, Sergey, Conrad, Thomas, Lührmann, Reinhard, Urlaub, Henning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2014
Materias:
Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923134/
https://www.ncbi.nlm.nih.gov/pubmed/24448447
http://dx.doi.org/10.1261/rna.041244.113

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923134/
https://www.ncbi.nlm.nih.gov/pubmed/24448447
http://dx.doi.org/10.1261/rna.041244.113

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