Cargando…

Kinotypes: stable species- and individual-specific profiles of cellular kinase activity

BACKGROUND: Recently, questions have been raised regarding the ability of animal models to recapitulate human disease at the molecular level. It has also been demonstrated that cellular kinases, individually or as a collective unit (the kinome), play critical roles in regulating complex biology. Des...

Descripción completa

Detalles Bibliográficos
Autores principales: Trost, Brett, Kindrachuk, Jason, Scruten, Erin, Griebel, Philip, Kusalik, Anthony, Napper, Scott
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3924188/
https://www.ncbi.nlm.nih.gov/pubmed/24314169
http://dx.doi.org/10.1186/1471-2164-14-854
_version_ 1782303713112621056
author Trost, Brett
Kindrachuk, Jason
Scruten, Erin
Griebel, Philip
Kusalik, Anthony
Napper, Scott
author_facet Trost, Brett
Kindrachuk, Jason
Scruten, Erin
Griebel, Philip
Kusalik, Anthony
Napper, Scott
author_sort Trost, Brett
collection PubMed
description BACKGROUND: Recently, questions have been raised regarding the ability of animal models to recapitulate human disease at the molecular level. It has also been demonstrated that cellular kinases, individually or as a collective unit (the kinome), play critical roles in regulating complex biology. Despite the intimate relationship between kinases and health, little is known about the variability, consistency and stability of kinome profiles across species and individuals. RESULTS: As a preliminary investigation of the existence of species- and individual-specific kinotypes (kinome signatures), peptide arrays were employed for the analysis of peripheral blood mononuclear cells collected weekly from human and porcine subjects (n = 6) over a one month period. The data revealed strong evidence for species-specific signalling profiles. Both humans and pigs also exhibited evidence for individual-specific kinome profiles that were independent of natural changes in blood cell populations. CONCLUSIONS: Species-specific kinotypes could have applications in disease research by facilitating the selection of appropriate animal models or by revealing a baseline kinomic signature to which treatment-induced profiles could be compared. Similarly, individual-specific kinotypes could have implications in personalized medicine, where the identification of molecular patterns or signatures within the kinome may depend on both the levels of kinome diversity and temporal stability across individuals.
format Online
Article
Text
id pubmed-3924188
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-39241882014-03-03 Kinotypes: stable species- and individual-specific profiles of cellular kinase activity Trost, Brett Kindrachuk, Jason Scruten, Erin Griebel, Philip Kusalik, Anthony Napper, Scott BMC Genomics Research Article BACKGROUND: Recently, questions have been raised regarding the ability of animal models to recapitulate human disease at the molecular level. It has also been demonstrated that cellular kinases, individually or as a collective unit (the kinome), play critical roles in regulating complex biology. Despite the intimate relationship between kinases and health, little is known about the variability, consistency and stability of kinome profiles across species and individuals. RESULTS: As a preliminary investigation of the existence of species- and individual-specific kinotypes (kinome signatures), peptide arrays were employed for the analysis of peripheral blood mononuclear cells collected weekly from human and porcine subjects (n = 6) over a one month period. The data revealed strong evidence for species-specific signalling profiles. Both humans and pigs also exhibited evidence for individual-specific kinome profiles that were independent of natural changes in blood cell populations. CONCLUSIONS: Species-specific kinotypes could have applications in disease research by facilitating the selection of appropriate animal models or by revealing a baseline kinomic signature to which treatment-induced profiles could be compared. Similarly, individual-specific kinotypes could have implications in personalized medicine, where the identification of molecular patterns or signatures within the kinome may depend on both the levels of kinome diversity and temporal stability across individuals. BioMed Central 2013-12-05 /pmc/articles/PMC3924188/ /pubmed/24314169 http://dx.doi.org/10.1186/1471-2164-14-854 Text en Copyright © 2013 Trost et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Trost, Brett
Kindrachuk, Jason
Scruten, Erin
Griebel, Philip
Kusalik, Anthony
Napper, Scott
Kinotypes: stable species- and individual-specific profiles of cellular kinase activity
title Kinotypes: stable species- and individual-specific profiles of cellular kinase activity
title_full Kinotypes: stable species- and individual-specific profiles of cellular kinase activity
title_fullStr Kinotypes: stable species- and individual-specific profiles of cellular kinase activity
title_full_unstemmed Kinotypes: stable species- and individual-specific profiles of cellular kinase activity
title_short Kinotypes: stable species- and individual-specific profiles of cellular kinase activity
title_sort kinotypes: stable species- and individual-specific profiles of cellular kinase activity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3924188/
https://www.ncbi.nlm.nih.gov/pubmed/24314169
http://dx.doi.org/10.1186/1471-2164-14-854
work_keys_str_mv AT trostbrett kinotypesstablespeciesandindividualspecificprofilesofcellularkinaseactivity
AT kindrachukjason kinotypesstablespeciesandindividualspecificprofilesofcellularkinaseactivity
AT scrutenerin kinotypesstablespeciesandindividualspecificprofilesofcellularkinaseactivity
AT griebelphilip kinotypesstablespeciesandindividualspecificprofilesofcellularkinaseactivity
AT kusalikanthony kinotypesstablespeciesandindividualspecificprofilesofcellularkinaseactivity
AT napperscott kinotypesstablespeciesandindividualspecificprofilesofcellularkinaseactivity