A GmRAV Ortholog Is Involved in Photoperiod and Sucrose Control of Flowering Time in Soybean

Photoperiod and sucrose levels play a key role in the control of flowering. GmRAV reflected a diurnal rhythm with the highest expression at 4 h after the beginning of a dark period in soybean leaves, and was highly up-regulated under short-day (SD) conditions, despite of not following a diurnal patt...

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Autores principales: Lu, Qingyao, Zhao, Lin, Li, Dongmei, Hao, Diqiu, Zhan, Yong, Li, Wenbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3925180/
https://www.ncbi.nlm.nih.gov/pubmed/24551235
http://dx.doi.org/10.1371/journal.pone.0089145
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author Lu, Qingyao
Zhao, Lin
Li, Dongmei
Hao, Diqiu
Zhan, Yong
Li, Wenbin
author_facet Lu, Qingyao
Zhao, Lin
Li, Dongmei
Hao, Diqiu
Zhan, Yong
Li, Wenbin
author_sort Lu, Qingyao
collection PubMed
description Photoperiod and sucrose levels play a key role in the control of flowering. GmRAV reflected a diurnal rhythm with the highest expression at 4 h after the beginning of a dark period in soybean leaves, and was highly up-regulated under short-day (SD) conditions, despite of not following a diurnal pattern under long-day (LD) conditions. GmRAV-i (GmRAV-inhibition) transgenic soybean exhibited early flowering phenotype. Two of the FT Arabidopsis homologs, GmFT2a and GmFT5a, were highly expressed in the leaves of soybeans with inhibition (-i) of GmRAV under SD conditions. Moreover, the transcript levels of the two FT homologs in GmRAV-i soybeans were more sensitive to SD conditions than LD conditions compared to the WT plant. GmRAV-i soybeans and Arabidopsis rav mutants showed more sensitive hypocotyl elongation responses when compared with wild-type seedlings, and GmRAV-ox overevpressed in tobacco revealed no sensitive changes in hypocotyl length. These indicated that GmRAV was a novel negative regulator of SD-mediated flowering and hypocotyl elongation. Although sucrose has been suggested to promote flowering induction in many plant species, high concentration of sucrose (4% [w/v]) applied into media defer flowering time in Arabidopsis wild-type and rav mutant. This delayed flowering stage might be caused by reduction of LEAFY expression. Furthermore, Arabidopsis rav mutants and GmRAV-i soybean plants were less sensitive to sucrose by the inhibition assays of hypocotyls and roots growth. In contrast, transgenic GmRAV overexpressing (-ox) tobacco plants displayed more sensitivity to sucrose. In conclusion, GmRAV was inferred to have a fundamental function in photoperiod, darkness, and sucrose signaling responses to regulate plant development and flowering induction.
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spelling pubmed-39251802014-02-18 A GmRAV Ortholog Is Involved in Photoperiod and Sucrose Control of Flowering Time in Soybean Lu, Qingyao Zhao, Lin Li, Dongmei Hao, Diqiu Zhan, Yong Li, Wenbin PLoS One Research Article Photoperiod and sucrose levels play a key role in the control of flowering. GmRAV reflected a diurnal rhythm with the highest expression at 4 h after the beginning of a dark period in soybean leaves, and was highly up-regulated under short-day (SD) conditions, despite of not following a diurnal pattern under long-day (LD) conditions. GmRAV-i (GmRAV-inhibition) transgenic soybean exhibited early flowering phenotype. Two of the FT Arabidopsis homologs, GmFT2a and GmFT5a, were highly expressed in the leaves of soybeans with inhibition (-i) of GmRAV under SD conditions. Moreover, the transcript levels of the two FT homologs in GmRAV-i soybeans were more sensitive to SD conditions than LD conditions compared to the WT plant. GmRAV-i soybeans and Arabidopsis rav mutants showed more sensitive hypocotyl elongation responses when compared with wild-type seedlings, and GmRAV-ox overevpressed in tobacco revealed no sensitive changes in hypocotyl length. These indicated that GmRAV was a novel negative regulator of SD-mediated flowering and hypocotyl elongation. Although sucrose has been suggested to promote flowering induction in many plant species, high concentration of sucrose (4% [w/v]) applied into media defer flowering time in Arabidopsis wild-type and rav mutant. This delayed flowering stage might be caused by reduction of LEAFY expression. Furthermore, Arabidopsis rav mutants and GmRAV-i soybean plants were less sensitive to sucrose by the inhibition assays of hypocotyls and roots growth. In contrast, transgenic GmRAV overexpressing (-ox) tobacco plants displayed more sensitivity to sucrose. In conclusion, GmRAV was inferred to have a fundamental function in photoperiod, darkness, and sucrose signaling responses to regulate plant development and flowering induction. Public Library of Science 2014-02-14 /pmc/articles/PMC3925180/ /pubmed/24551235 http://dx.doi.org/10.1371/journal.pone.0089145 Text en © 2014 Lu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Lu, Qingyao
Zhao, Lin
Li, Dongmei
Hao, Diqiu
Zhan, Yong
Li, Wenbin
A GmRAV Ortholog Is Involved in Photoperiod and Sucrose Control of Flowering Time in Soybean
title A GmRAV Ortholog Is Involved in Photoperiod and Sucrose Control of Flowering Time in Soybean
title_full A GmRAV Ortholog Is Involved in Photoperiod and Sucrose Control of Flowering Time in Soybean
title_fullStr A GmRAV Ortholog Is Involved in Photoperiod and Sucrose Control of Flowering Time in Soybean
title_full_unstemmed A GmRAV Ortholog Is Involved in Photoperiod and Sucrose Control of Flowering Time in Soybean
title_short A GmRAV Ortholog Is Involved in Photoperiod and Sucrose Control of Flowering Time in Soybean
title_sort gmrav ortholog is involved in photoperiod and sucrose control of flowering time in soybean
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3925180/
https://www.ncbi.nlm.nih.gov/pubmed/24551235
http://dx.doi.org/10.1371/journal.pone.0089145
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