Rapid Sanger Sequencing of the 16S rRNA Gene for Identification of Some Common Pathogens

Conventional Sanger sequencing remains time-consuming and laborious. In this study, we developed a rapid improved sequencing protocol of 16S rRNA for pathogens identification by using a new combination of SYBR Green I real-time PCR and Sanger sequencing with FTA® cards. To compare the sequencing qua...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Linxiang, Cai, Ying, Zhou, Guangbiao, Shi, Xiaojun, Su, Jianhui, Chen, Guanwu, Lin, Kun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3925228/
https://www.ncbi.nlm.nih.gov/pubmed/24551186
http://dx.doi.org/10.1371/journal.pone.0088886

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3925228/
https://www.ncbi.nlm.nih.gov/pubmed/24551186
http://dx.doi.org/10.1371/journal.pone.0088886

Ejemplares similares