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Rapid Sanger Sequencing of the 16S rRNA Gene for Identification of Some Common Pathogens

Conventional Sanger sequencing remains time-consuming and laborious. In this study, we developed a rapid improved sequencing protocol of 16S rRNA for pathogens identification by using a new combination of SYBR Green I real-time PCR and Sanger sequencing with FTA® cards. To compare the sequencing qua...

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Detalles Bibliográficos
Autores principales:	Chen, Linxiang, Cai, Ying, Zhou, Guangbiao, Shi, Xiaojun, Su, Jianhui, Chen, Guanwu, Lin, Kun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3925228/ https://www.ncbi.nlm.nih.gov/pubmed/24551186 http://dx.doi.org/10.1371/journal.pone.0088886

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