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Genome sequencing and analysis of the paclitaxel-producing endophytic fungus Penicillium aurantiogriseum NRRL 62431

BACKGROUND: Paclitaxel (Taxol™) is an important anticancer drug with a unique mode of action. The biosynthesis of paclitaxel had been considered restricted to the Taxus species until it was discovered in Taxomyces andreanae, an endophytic fungus of T. brevifolia. Subsequently, paclitaxel was found i...

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Autores principales: Yang, Yanfang, Zhao, Hainan, Barrero, Roberto A, Zhang, Baohong, Sun, Guiling, Wilson, Iain W, Xie, Fuliang, Walker, Kevin D, Parks, Joshua W, Bruce, Robert, Guo, Guangwu, Chen, Li, Zhang, Yong, Huang, Xin, Tang, Qi, Liu, Hongwei, Bellgard, Matthew I, Qiu, Deyou, Lai, Jinsheng, Hoffman, Angela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3925984/
https://www.ncbi.nlm.nih.gov/pubmed/24460898
http://dx.doi.org/10.1186/1471-2164-15-69
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author Yang, Yanfang
Zhao, Hainan
Barrero, Roberto A
Zhang, Baohong
Sun, Guiling
Wilson, Iain W
Xie, Fuliang
Walker, Kevin D
Parks, Joshua W
Bruce, Robert
Guo, Guangwu
Chen, Li
Zhang, Yong
Huang, Xin
Tang, Qi
Liu, Hongwei
Bellgard, Matthew I
Qiu, Deyou
Lai, Jinsheng
Hoffman, Angela
author_facet Yang, Yanfang
Zhao, Hainan
Barrero, Roberto A
Zhang, Baohong
Sun, Guiling
Wilson, Iain W
Xie, Fuliang
Walker, Kevin D
Parks, Joshua W
Bruce, Robert
Guo, Guangwu
Chen, Li
Zhang, Yong
Huang, Xin
Tang, Qi
Liu, Hongwei
Bellgard, Matthew I
Qiu, Deyou
Lai, Jinsheng
Hoffman, Angela
author_sort Yang, Yanfang
collection PubMed
description BACKGROUND: Paclitaxel (Taxol™) is an important anticancer drug with a unique mode of action. The biosynthesis of paclitaxel had been considered restricted to the Taxus species until it was discovered in Taxomyces andreanae, an endophytic fungus of T. brevifolia. Subsequently, paclitaxel was found in hazel (Corylus avellana L.) and in several other endophytic fungi. The distribution of paclitaxel in plants and endophytic fungi and the reported sequence homology of key genes in paclitaxel biosynthesis between plant and fungi species raises the question about whether the origin of this pathway in these two physically associated groups could have been facilitated by horizontal gene transfer. RESULTS: The ability of the endophytic fungus of hazel Penicillium aurantiogriseum NRRL 62431 to independently synthesize paclitaxel was established by liquid chromatography-mass spectrometry and proton nuclear magnetic resonance. The genome of Penicillium aurantiogriseum NRRL 62431 was sequenced and gene candidates that may be involved in paclitaxel biosynthesis were identified by comparison with the 13 known paclitaxel biosynthetic genes in Taxus. We found that paclitaxel biosynthetic gene candidates in P. aurantiogriseum NRRL 62431 have evolved independently and that horizontal gene transfer between this endophytic fungus and its plant host is unlikely. CONCLUSIONS: Our findings shed new light on how paclitaxel-producing endophytic fungi synthesize paclitaxel, and will facilitate metabolic engineering for the industrial production of paclitaxel from fungi.
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spelling pubmed-39259842014-02-18 Genome sequencing and analysis of the paclitaxel-producing endophytic fungus Penicillium aurantiogriseum NRRL 62431 Yang, Yanfang Zhao, Hainan Barrero, Roberto A Zhang, Baohong Sun, Guiling Wilson, Iain W Xie, Fuliang Walker, Kevin D Parks, Joshua W Bruce, Robert Guo, Guangwu Chen, Li Zhang, Yong Huang, Xin Tang, Qi Liu, Hongwei Bellgard, Matthew I Qiu, Deyou Lai, Jinsheng Hoffman, Angela BMC Genomics Research Article BACKGROUND: Paclitaxel (Taxol™) is an important anticancer drug with a unique mode of action. The biosynthesis of paclitaxel had been considered restricted to the Taxus species until it was discovered in Taxomyces andreanae, an endophytic fungus of T. brevifolia. Subsequently, paclitaxel was found in hazel (Corylus avellana L.) and in several other endophytic fungi. The distribution of paclitaxel in plants and endophytic fungi and the reported sequence homology of key genes in paclitaxel biosynthesis between plant and fungi species raises the question about whether the origin of this pathway in these two physically associated groups could have been facilitated by horizontal gene transfer. RESULTS: The ability of the endophytic fungus of hazel Penicillium aurantiogriseum NRRL 62431 to independently synthesize paclitaxel was established by liquid chromatography-mass spectrometry and proton nuclear magnetic resonance. The genome of Penicillium aurantiogriseum NRRL 62431 was sequenced and gene candidates that may be involved in paclitaxel biosynthesis were identified by comparison with the 13 known paclitaxel biosynthetic genes in Taxus. We found that paclitaxel biosynthetic gene candidates in P. aurantiogriseum NRRL 62431 have evolved independently and that horizontal gene transfer between this endophytic fungus and its plant host is unlikely. CONCLUSIONS: Our findings shed new light on how paclitaxel-producing endophytic fungi synthesize paclitaxel, and will facilitate metabolic engineering for the industrial production of paclitaxel from fungi. BioMed Central 2014-01-25 /pmc/articles/PMC3925984/ /pubmed/24460898 http://dx.doi.org/10.1186/1471-2164-15-69 Text en Copyright © 2014 Yang et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Yang, Yanfang
Zhao, Hainan
Barrero, Roberto A
Zhang, Baohong
Sun, Guiling
Wilson, Iain W
Xie, Fuliang
Walker, Kevin D
Parks, Joshua W
Bruce, Robert
Guo, Guangwu
Chen, Li
Zhang, Yong
Huang, Xin
Tang, Qi
Liu, Hongwei
Bellgard, Matthew I
Qiu, Deyou
Lai, Jinsheng
Hoffman, Angela
Genome sequencing and analysis of the paclitaxel-producing endophytic fungus Penicillium aurantiogriseum NRRL 62431
title Genome sequencing and analysis of the paclitaxel-producing endophytic fungus Penicillium aurantiogriseum NRRL 62431
title_full Genome sequencing and analysis of the paclitaxel-producing endophytic fungus Penicillium aurantiogriseum NRRL 62431
title_fullStr Genome sequencing and analysis of the paclitaxel-producing endophytic fungus Penicillium aurantiogriseum NRRL 62431
title_full_unstemmed Genome sequencing and analysis of the paclitaxel-producing endophytic fungus Penicillium aurantiogriseum NRRL 62431
title_short Genome sequencing and analysis of the paclitaxel-producing endophytic fungus Penicillium aurantiogriseum NRRL 62431
title_sort genome sequencing and analysis of the paclitaxel-producing endophytic fungus penicillium aurantiogriseum nrrl 62431
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3925984/
https://www.ncbi.nlm.nih.gov/pubmed/24460898
http://dx.doi.org/10.1186/1471-2164-15-69
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