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Selection of Suitable Reference Genes for Normalization of Quantitative Real-Time Polymerase Chain Reaction in Human Cartilage Endplate of the Lumbar Spine

The quantitative real-time polymerase chain reaction (qRT-PCR) is one of the most widely used methods to study gene expression profiles, and it requires appropriate normalization for accurate and reliable results. Although several genes are commonly used as reference genes (such as GAPDH, ACTB, and...

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Detalles Bibliográficos
Autores principales: Zhou, Zhi-Jie, Zhang, Jian-Feng, Xia, Ping, Wang, Ji-Ying, Chen, Shuai, Fang, Xiang-Qian, Fan, Shun-Wu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3928306/
https://www.ncbi.nlm.nih.gov/pubmed/24558443
http://dx.doi.org/10.1371/journal.pone.0088892