Antibody-drug conjugate model fast characterization by LC-MS following IdeS proteolytic digestion

Here we report the design and production of an antibody-fluorophore conjugate (AFC) as a non-toxic model of an antibody-drug conjugate (ADC). This AFC is based on the conjugation of dansyl sulfonamide ethyl amine (DSEA)-linker maleimide on interchain cysteines of trastuzumab used as a reference anti...

Descripción completa

Detalles Bibliográficos
Autores principales: Wagner-Rousset, Elsa, Janin-Bussat, Marie-Claire, Colas, Olivier, Excoffier, Melissa, Ayoub, Daniel, Haeuw, Jean-François, Rilatt, Ian, Perez, Michel, Corvaïa, Nathalie, Beck, Alain
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2014
Materias:
Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3929440/
https://www.ncbi.nlm.nih.gov/pubmed/24135617
http://dx.doi.org/10.4161/mabs.26773

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3929440/
https://www.ncbi.nlm.nih.gov/pubmed/24135617
http://dx.doi.org/10.4161/mabs.26773

Ejemplares similares