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Antibody-drug conjugate model fast characterization by LC-MS following IdeS proteolytic digestion

Here we report the design and production of an antibody-fluorophore conjugate (AFC) as a non-toxic model of an antibody-drug conjugate (ADC). This AFC is based on the conjugation of dansyl sulfonamide ethyl amine (DSEA)-linker maleimide on interchain cysteines of trastuzumab used as a reference anti...

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Detalles Bibliográficos
Autores principales:	Wagner-Rousset, Elsa, Janin-Bussat, Marie-Claire, Colas, Olivier, Excoffier, Melissa, Ayoub, Daniel, Haeuw, Jean-François, Rilatt, Ian, Perez, Michel, Corvaïa, Nathalie, Beck, Alain
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Landes Bioscience 2014
Materias:	Report
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3929440/ https://www.ncbi.nlm.nih.gov/pubmed/24135617 http://dx.doi.org/10.4161/mabs.26773

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