Potential Pitfalls and Solutions for Use of Fluorescent Fusion Proteins to Study the Lysosome

Use of fusion protein tags to investigate lysosomal proteins can be complicated by the acidic, protease-rich environment of the lysosome. Potential artifacts include degradation or release of the tag and acid quenching of fluorescence. Tagging can also affect protein folding, glycosylation and/or tr...

Descripción completa

Detalles Bibliográficos
Autores principales: Huang, Ling, Pike, Douglas, Sleat, David E., Nanda, Vikas, Lobel, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3931630/
https://www.ncbi.nlm.nih.gov/pubmed/24586430
http://dx.doi.org/10.1371/journal.pone.0088893

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3931630/
https://www.ncbi.nlm.nih.gov/pubmed/24586430
http://dx.doi.org/10.1371/journal.pone.0088893

Ejemplares similares